PXD059975-1

PXD059975 is an original dataset announced via ProteomeXchange.

Title	A priority code in mitochondrial presequences - Data set 2: Proteome of WT and CNS1 mutant yeast strains
Description	The biogenesis of mitochondria relies on the import of hundreds of different precursor proteins from the cytosol. Most of these proteins are synthesized with N-terminal presequences which serve as mitochondrial targeting signals. Presequences consistently form amphipathic helices, but they considerably differ in respect to their primary structure and length. Here we show that presequences can be classified into seven different groups on basis of their specific features. Using a test set of different presequences, we observed that group A presequences endow precursor proteins with improved in vitro import characteristics. We developed IQ-compete, a novel assay based on fluorescence de-quenching to monitor the import efficiencies of mitochondrial precursors in vivo. With this assay, we confirmed the increased import competence of group A presequences. Using mass spectrometry, we found that the presequence of the group A protein Oxa1 specifically recruits the tetratricopeptide repeat (TPR) containing protein TOMM34 to the cytosolic precursor protein. TOMM34 apparently serves as presequence-specific targeting factor which increases the import efficiency of a specific subset of mitochondrial precursor proteins. Our results suggest that presequences contain a protein-specific priority code that encrypts the targeting mechanism of individual mitochondrial precursor proteins.
HostingRepository	PRIDE
AnnounceDate	2025-07-09
AnnouncementXML	Submission_2025-07-09_10:23:39.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-20 06:35:00	ID requested
⏵ 1	2025-07-09 10:23:40	announced

Dataset with its publication pending

submitter keyword: mitochondria,yeast

Markus Räschle
contact affiliation	Molecular Genetics, RPTU Kaiserslautern, Germany
contact email	raeschle@rhrk.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059975

PRIDE project URI

• PRIDE
  1. PXD059975
     1. Label: PRIDE project
     2. Name: A priority code in mitochondrial presequences - Data set 2: Proteome of WT and CNS1 mutant yeast strains