PXD059963 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Metformin Protects Against Persistent Atrial Fibrillation in an Equine Model |
| Description | Background: Horses are one of the few animals that spontaneously develop atrial fibrillation (AF), making them a powerful model for studying AF mechanisms and treatment effects. Despite the initial effectiveness of treatment in both horses and humans, AF-induced atrial remodelling compromises long-term success. Observational studies have suggested that metformin reduces the risk of AF, but its effects on progressive AF-induced atrial remodelling have not yet been evaluated in a high-fidelity large animal model. Methods: Here, we employed a longitudinal horse model of tachypacing-induced self-sustained AF to characterize the electrical, molecular, and metabolic atrial changes over four months of disease, with and without metformin treatment. Electrophysiological and multi-omic approaches were combined with histology, echocardiography, biochemical and mitochondrial analyses to evaluate disease progression and treatment response. Results: The horse model replicated critical aspects of AF-induced atrial remodelling observed in humans, including electrical and structural changes. Despite upregulation of metabolic genes and proteins in AF, no significant ultrastructural mitochondrial changes were detected. Metformin-treated horses were less susceptible to AF and sustained a less complex AF substrate in the right atrium after four months of disease. These effects were associated with increased activity of the metabolic regulator, AMP-activated kinase (AMPK) , changes in metabolic pathways and modulation of ion-channel gene expression. Metformin did not appear to alter left atrial substrate remodelling. Conclusion: Metformin treatment conferred electrical protection in both the early and later stages of AF in a translationally relevant preclinical model. These findings support metformin as a lead molecul ar for AF-prevention, warranting further mechanistic and clinical studies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-03 |
| AnnouncementXML | Submission_2025-12-03_10:13:10.157.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Joakim Bastrup |
| SpeciesList | scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-20 01:51:42 | ID requested | |
| ⏵ 1 | 2025-12-03 10:13:10 | announced | |
Publication List
Keyword List
| submitter keyword: electrophysiology, large animal model, atrial remodelling,AMPK, translational cardiology |
Contact List
| Thomas Jepps |
|---|
| contact affiliation | Department of Biomedical Sciences, Jepps lab, University of Copenhagen, Denmark |
| contact email | tjepps@sund.ku.dk |
| lab head | |
| Joakim Bastrup |
|---|
| contact affiliation | Department of Biomedical Sciences, University of Copenhagen, Denmark |
| contact email | joakimbastrup@hotmail.dk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059963
- Label: PRIDE project
- Name: Metformin Protects Against Persistent Atrial Fibrillation in an Equine Model
