PXD059960-1

PXD059960 is an original dataset announced via ProteomeXchange.

Title	Trifunctional lipid derivatives: PEs mi-tochondrial interactome
Description	In this study, we employed chemically synthesized trifunctional lipid probes to identify putative protein partners that interact with lipids in HeLa cells. These probes incorporate three distinct chemical functionalities. First, a coumarin-based “caging” moiety is added to the lipid head group, which shields the lipid from metabolic degradation and facilitates its uptake by cells. Second, a diazirine group is embedded within the acyl chain. Upon photoactivation (e.g., exposure to UV light), this diazirine crosslinks the lipid probe to nearby proteins. Finally, an alkyne group is introduced at the tail of the acyl chain, enabling click chemistry conjugation to azide-functionalized beads.This permits the selective enrichment of crosslinked protein complexes for downstream isolation and mass spectrometry–based proteomic analysis. This strategy allows the identification of native lipid–protein interactions while minimally perturbing cellular systems. Using HeLa cells as our experimental model, we were able to detect and enrich candidate lipid-binding proteins for subsequent in-depth proteomic characterization.
HostingRepository	PRIDE
AnnounceDate	2025-01-21
AnnouncementXML	Submission_2025-01-20_23:46:33.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-20 00:47:33	ID requested
⏵ 1	2025-01-20 23:46:33	announced

10.1039/D4CC03599B;

submitter keyword: phosphatidic-acid, trifunctional lipid, phospholipid, phosphatidylinositol, phosphatidylethanolamine,lipid interactome

Carsten Schultz
contact affiliation	Professor of Chemical Physiology and Biochemistry, School of Medicine at OHSU Chair, Chemical Physiology and Biochemistry, School of Medicine Helen Jo Whitsell Dean's Leadership Professor Graduate Program in Biomedical Sciences, School of Medicine
contact email	schulcar@ohsu.edu
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD059960

PRIDE project URI

• PRIDE
  1. PXD059960
     1. Label: PRIDE project
     2. Name: Trifunctional lipid derivatives: PEs mi-tochondrial interactome