PXD059956-1

PXD059956 is an original dataset announced via ProteomeXchange.

Title	Monocyte histone lactylation-regulated exosomal periostin promotes retinal neovascularization
Description	Exosomes serve as signaling messengers that facilitate intercellular communication through delivering molecular cargo. Here, we observed elevated levels of exosomal periostin (POSTN) in blood plasma of patients with proliferative diabetic retinopathy (PDR). Monocytes-derived exosomal POSTN was increased under high glucose condition, and deletion of Postn in myeloid cells reduced retinal neovascularization. Monocytes isolated from the blood of PDR patients exhibited heightened glycolytic activity and elevated histone lactylation levels, particularly H4K8 lactylation (H4K8la). Knockout of Hexokinase 2 (Hk2) in myeloid cells led to reduced H4K8la and POSTN levels and inhibited retinal neovascularization. Exogenous exosomal POSTN partially reversed the angiogenic defects caused by Postn or Hk2 deletion in myeloid cells. Mechanistically, exosomal POSTN stabilized hypoxia-inducible factor 1-alpha (HIF1-α) and upregulated the expression of angiogenic genes. Notably, treatment with metformin reduced retinal neovascularization by decreasing monocytes glycolysis and lowering exosomal POSTN levels. In summary, these findings underscore the critical role of circulating exosomal POSTN in retinal neovascularization and highlight its potential as a therapeutic target for angiogenic retinopathies.
HostingRepository	iProX
AnnounceDate	2025-01-20
AnnouncementXML	Submission_2025-01-19_23:45:48.247.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haorui Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-19 23:45:30	ID requested
⏵ 1	2025-01-19 23:45:48	announced

Dataset with its publication pending

submitter keyword: Monocyte histone lactylation-regulated exosomal periostin promotes retinal neovascularization

Hongyuan Song
contact affiliation	Changhai Hospital
contact email	johnsoung@hotmail.com
lab head
Haorui Zhang
contact affiliation	Changhai Hospital
contact email	stephenzhr123@163.com
dataset submitter

iProX dataset URI