PXD059945-1

PXD059945 is an original dataset announced via ProteomeXchange.

Title	Proline-adjacent phosphosites on S. cerevisiae histone demethylase Rph1p are salt stress responsive and important for cell growth in the salt stress response
Description	Phosphorylation of histone lysine demethylases is an important mechanism by which the cell modulates chromatin dynamics to regulate its response to stress. There is evidence that the Saccharomyces cerevisiae H3K36me2/3 demethylase, Rph1p, is an integrator of many signalling events. However, there is limited understanding of how most Rph1p phosphosites affect or reflect its role in stress response pathways and how this modulates a cellular response. Here, we investigated the role of Rph1p phosphorylation in the salt stress response. We showed that Rph1p is extensively phosphorylated in response to acute high salt stress, particularly at proline-adjacent residues. Genomic phosphonull mutations identified a subset of salt-stress responsive phosphosites – S410, T411, S412 and S689 – that are important for yeast cell growth in this condition. However, these sites were not associated with gross changes in H3K36 methylation, suggesting that phosphorylation at specific sites may instead direct Rph1p to specific regions of the chromatin in the salt stress response. Proteomic investigation of slow growing Rph1p-S412A and Rph1p-S689A mutants revealed functional enrichment for proteins involved in cell structure and morphology, as well as mitochondria-associated proteins. These findings show that phosphorylation of Rph1p at S412 and S689 is important for modulating the yeast cell’s response to salt stress, offering insights into stress-responsive regulatory mechanisms in all eukaryotic cells.
HostingRepository	PRIDE
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-02_18:53:17.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicola Karakatsanis
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-19 17:30:33	ID requested
⏵ 1	2025-11-02 18:53:18	announced

Karakatsanis NM, Hamey JJ, Wilkins MR, Proline-Adjacent Phosphosites on Saccharomyces cerevisiae Histone Demethylase Rph1p are Salt Stress Responsive and Important for Cell Growth Under Salt Stress. Mol Cell Proteomics, 24(10):101066(2025) [pubmed]

10.1016/j.mcpro.2025.101066;

submitter keyword: LC-MSMS,Yeast, methylation, demethylase, phosphorylation

Marc R
contact affiliation	Systems Biology Initiative, School of Biotechnology & Biomolecular Sciences, UNSW Sydney, Australia
contact email	m.wilkins@unsw.edu.au
lab head
Nicola Karakatsanis
contact affiliation	UNSW
contact email	z5302209@ad.unsw.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059945
     1. Label: PRIDE project
     2. Name: Proline-adjacent phosphosites on S. cerevisiae histone demethylase Rph1p are salt stress responsive and important for cell growth in the salt stress response