PXD059938-1

PXD059938 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of invasive breast cancer cells treated with CBD reveals new proteins associated with the reversal of their Epithelial-Mesenchymal Transition
Description	Cannabidiol (CBD) has been used in the treatment of cancer types associated with an inflammatory microenvironment. Although it has been demonstrated that CBD blocks and reverses the epithelial-mesenchymal-transition (EMT) induced by IL-1β, there is limited understanding of how CBD regulates these processes. MCF-7 cells were used to obtain the aggressive phenotype called 6D cells, CBD was then applied to reverse the EMT, to restore a benign epithelial phenotype. In this work, the protein expression in cells going through these processes was analyzed by mass spectrometry. By comparing proteomes of 6D vs. MCF-7 cells, as well as the proteomes of 6D+CBD cells vs. 6D cells or MCF-7 cells, it was foud that GOLGA2, KRT16, and RPRD2 expression was up-regulated by IL-1β and subsequently down-regulated by CBD, and KLHDC7B protein was repressed by IL-1β, and induced by CBD. These observations suggest that these four proteins could represent points of crosstalk between the IL-1β and CBD signaling pathways. Additionally, two protein networks were predicted; one associated with EMT including the proteins BRCA1, MSN, and CORO1A; and other associated with the CBD signaling, consisting of proteins SUPT16H, SETD2, and H2BC12, suggesting that CBD restores the epithelial morphology epigenetically and highlights potential targets for anticancer therapy.
HostingRepository	PRIDE
AnnounceDate	2025-05-16
AnnouncementXML	Submission_2025-05-16_10:34:31.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LAZARO GARCIA
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-18 13:38:09	ID requested
⏵ 1	2025-05-16 10:34:31	announced

10.3390/IJMS26104721;

submitter keyword: Breast cancer

Cannabidiol

Epithelial-mesenchymal-transition

proteomics

Isaura Meza
contact affiliation	Department of Molecular Biomedicine, CINVESTAV-IPN
contact email	imeza@cinvestav.mx
lab head
LAZARO GARCIA
contact affiliation	Molecular Biomedicine Department, CINVESTAV
contact email	lazaro.garcia@cinvestav.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059938
     1. Label: PRIDE project
     2. Name: Proteomic analysis of invasive breast cancer cells treated with CBD reveals new proteins associated with the reversal of their Epithelial-Mesenchymal Transition