PXD059899 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global surface proteomes of hPSC-derived brain microvascular endothelial-like cells (hPSC-BMELCs), human cerebral microvascular endothelial cell line D3 (HCMEC/D3), and human umbilical vein endothelial cells (HUVECs) |
| Description | Abundance proteomics analysis was performed to characterize the surface proteome of three cell lines: hPSC-BMELCs, a stem cell derived model for human blood-brain barrier endothelial cells; HCMEC/D3, an immortalized cell line of brain microvascular endothelial cells; and HUVECs, primary human endothelial cells isolated from the umbilical vein. The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit was used which enables biotinylation and isolation of cell surface proteins by labeling with EZ-Link Sulfo-NHS-SS-Biotin, a thiol-cleavable amine-reactive biotinylation reagent, followed by capture with NeutrAvidin Agarose and reduction of the disulfide bond with DTT for protein release. Technical replicates of cells treated with biotin and without biotin were included for each cell line. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-09_16:30:37.978.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mehdi Bouhaddou |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-16 16:23:37 | ID requested | |
| ⏵ 1 | 2025-11-09 16:30:38 | announced | |
Publication List
Keyword List
| submitter keyword: Abundance proteomics, receptors, endothelial cells, surface proteome |
Contact List
| Mehdi Bouhaddou |
|---|
| contact affiliation | University of California Los Angeles |
| contact email | mehdibouhaddou@gmail.com |
| lab head | |
| Mehdi Bouhaddou |
|---|
| contact affiliation | UCLA |
| contact email | bouhaddoulab@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD059899 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059899
- Label: PRIDE project
- Name: Global surface proteomes of hPSC-derived brain microvascular endothelial-like cells (hPSC-BMELCs), human cerebral microvascular endothelial cell line D3 (HCMEC/D3), and human umbilical vein endothelial cells (HUVECs)
