PXD059881 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CIP2A mediates mitotic recruitment of SLX4/MUS81/XPF to replication stress-induced DNA lesions to maintain genome integrity |
| Description | Perturbed DNA replication can result in genomic regions that remain incompletely replicated at the point that cells enter mitosis. Transmission of replication-mediated DNA lesions into mitosis may prevent proper chromosomes segregation, and cells therefore require mechanisms to resolve these lesions during mitosis. CIP2A in conjunction with TOPBP1 has been described to function as a molecular tether to connect broken DNA molecules during mitosis, but a role for CIP2A in processing of incompletely replicated DNA has remained unclear. We find that the CIP2A-TOPBP1 complex forms large filamentous structures at sites of at sites of mitotic DNA synthesis during mitosis. Using endogenous IP we aimed to identify CIP2A-interactions induced by irradiation or replication inhibition, and find that CIP2A recruits members the SMX tri-nuclease complex, including SLX4, MUS81 and ERCC1/XPF. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-24 |
| AnnouncementXML | Submission_2025-10-24_05:13:50.013.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rolf de Boer |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-16 06:55:13 | ID requested | |
| ⏵ 1 | 2025-10-24 05:13:50 | announced | |
Publication List
Keyword List
Contact List
| Marcel van |
|---|
| contact affiliation | Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713GZ, Groningen, the Netherlands (lab head) |
| contact email | m.vugt@umcg.nl |
| lab head | |
| Rolf de Boer |
|---|
| contact affiliation | University Medical Center Groningen |
| contact email | h.r.de.boer@umcg.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD059881 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059881
- Label: PRIDE project
- Name: CIP2A mediates mitotic recruitment of SLX4/MUS81/XPF to replication stress-induced DNA lesions to maintain genome integrity
