PXD059871-1

PXD059871 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL
Description	Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF Pro 2 mass spectrometer (Bruker Daltonics) in data-dependent mode with parallel accumulation serial fragmentation (PASEF) enabled. The raw data was analyzed using Fragpipe (v 22.0). MS/MS spectra were searched using MSFragger (v 4.1) against forward and reversed Homo sapiens UniProt sequence database (UP000005640) supplemented with common contaminants (total 20,979 sequences excluding decoys).
HostingRepository	MassIVE
AnnounceDate	2025-06-05
AnnouncementXML	Submission_2025-06-05_21:16:44.532.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikhil Dev Narendradev
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-16 02:32:50	ID requested
⏵ 1	2025-06-05 21:16:44	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: RFFL, Interactome, E3 ligase, Substrates, DatasetType:Proteomics

submitter keyword: RFFL, Interactome, E3 ligase, Substrates, DatasetType:Proteomics

Contact List

Prof. S. Murty Srinivasula
contact affiliation	Indian Institute of Science Education and Research Thiruvananthapuram
contact email	sms@iisertvm.ac.in
lab head
Nikhil Dev Narendradev
contact affiliation	Indian Institute of Science Education and Research Thiruvananthapuram
contact email	nikhilndev18@iisertvm.ac.in
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000096868/