PXD059856-1

PXD059856 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Sternaspis chinensis to understand phosphate biomineralization in annelids
Description	Organisms with phosphate hard tissues contribute to the evolutionary history of life and global biogeochemical cycles. The annelid Sternaspidae is an important group of phosphate-mineralizing organisms, knowns for its unique amorphous phosphate ventro-caudal shield. However, the molecular mechanisms underlying the formation of their phosphate shields remain unclear. In this study, we identified shield matrix proteins (ShiMPs) from Sternaspis chinensis (SC group with harder shield) and Sternaspis liui syn (SL group with soft shield) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 1,360 ShiMPs were identified, with 237 differentially expressed proteins between the two morphologies. The differential expression of collagen, extracellular matrix proteins with new EGF-VWA domains, and calcium-binding proteins in different morphological shields suggested that they may be associated with the variation in shield phenotypes. Furthermore, the phenoloxidase innate immune cascade pathway was found to be upregulated in the SL group. Compared with other biomineral proteins, sternaspid shields lacked typical chitin-related proteins but were rich in collagen proteins containing EGF domains, suggesting that collagen may serve as a scaffold for amorphous phosphate deposition. This study provided a molecular dataset for the formation of sternaspid shields and revealed the molecular regulatory features of different shield morphologies.
HostingRepository	iProX
AnnounceDate	2025-01-16
AnnouncementXML	Submission_2025-01-15_22:19:01.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meiling Ge
SpeciesList	scientific name: Sternaspis chinensis; NCBI TaxID: 2607890;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 22:18:15	ID requested
⏵ 1	2025-01-15 22:19:01	announced

Dataset with its publication pending

submitter keyword: Sternaspidae, Phosphate, Biomineralization, Shield matrix protein, Collagen

Xuelei Zhang
contact affiliation	First Institute of Oceanography, Ministry of Natural Resources
contact email	zhangxl@fio.org.cn
lab head
Meiling Ge
contact affiliation	First Institute of Oceanography, Ministry of Natural Resources
contact email	geml1208@163.com
dataset submitter

iProX dataset URI