PXD059838-1

PXD059838 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Interaction proteins with KAT6A
Description	Lysine acetyltransferases (KATs) catalyze the transfer of acetyl groups from acetyl-CoA to lysine residues. Our proteomic analysis revealed that KAT6A is acetylated at lysine 815 (K815), corresponding to mouse K816, in the hearts of mice fed a ketogenic diet under high blood pressure. To identify changes in KAT6A interaction partners by K815 acetylation in cardiomyocytes, we performed LC-MS/MS proteomics. We transfected adenovirus-carrying FLAG-KAT6A-wild type (WT, Control (Ctrl)), -K815R (an acetylation resistant mutant, KR), and -K815Q (an acetylation mimetic mutant, KQ) into cardiomyocytes for 2 days. Adenovirus-carrying FLAG was used as a control. KAT6A was immunoprecipitated with anti-FLAG affinity beads, followed by washing, competitive elution with 3x FLAG peptides, and separation by SDS-PAGE.
HostingRepository	PRIDE
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-02_17:04:22.637.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059838
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michinari Nakamura
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 14:05:42	ID requested
⏵ 1	2025-11-02 17:04:23	announced

Publication List

10.6019/PXD059838;
10.1016/j.molmet.2025.102239;
Keller MA, Ivessa A, Liu T, Li H, Romanienko PJ, Nakamura M, KAT6A acetylation regulates AMPK function and hypertrophic remodeling in the heart. Mol Metab, 101():102239(2025) [pubmed]

10.6019/PXD059838;

10.1016/j.molmet.2025.102239;

Keller MA, Ivessa A, Liu T, Li H, Romanienko PJ, Nakamura M, KAT6A acetylation regulates AMPK function and hypertrophic remodeling in the heart. Mol Metab, 101():102239(2025) [pubmed]

Keyword List

submitter keyword: Lysine acetyltransferase,KAT6A, Cardiomyocyte

submitter keyword: Lysine acetyltransferase,KAT6A, Cardiomyocyte

Contact List

Michinari Nakamura
contact affiliation	Department of Cell Biology and Molecular Medicine Rutgers New Jersey Medical School Rutgers Biomedical and Health Sciences Rutgers The State University of New Jersey
contact email	nakamumi@njms.rutgers.edu
lab head
Michinari Nakamura
contact affiliation	Rutgers
contact email	nakamumi@njms.rutgers.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD059838
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD059838

PRIDE project URI

Repository Record List

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