PXD059833-1

PXD059833 is an original dataset announced via ProteomeXchange.

Title	TgCoq5 proximity proteome in mitochondria and cytosolic fractions using FbxO14 as spatial reference
Description	Coenzyme Q or Ubiquinone (UQ) is an essential electron carrier and antioxidant. In yeast and mammals, several enzymes involved in the UQ synthesis form a protein complex in the mitochondrial matrix. Blast searches of the Toxoplasma gondii proteome failed to reveal several essential enzymes of the ubiquinone synthesis pathway. With the aim to identify those missing enzymes, we generated mutant strain expressing TurboID at the C-terminus of TgCoq5, one of the enzymes we have validated to be involved in UQ synthesis in T. gondii. Using a strategy combining biotinylation of TgCoq5-TurboID and FbxO14-TurboID, used as spatial reference, and subcellular fractionation. We investigated the proximal proteome of TgCoq5 and FbxO14 in the mitochondrial enriched fractions, P2, and cytosolic enriched fractions, S4.
HostingRepository	PRIDE
AnnounceDate	2025-07-31
AnnouncementXML	Submission_2025-07-31_11:43:27.065.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Baihetiya Baierna
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	biotinylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 12:11:02	ID requested
⏵ 1	2025-07-31 11:43:27	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii, TurboID, mitochondria

Silvia Moreno
contact affiliation	Center for Tropical and Emerging Global Diseases; Department of Cellular Biology, University of Georgia, Athens, Georgia, USA
contact email	smoreno@uga.edu
lab head
Baihetiya Baierna
contact affiliation	UGA
contact email	bb81719@uga.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059833
     1. Label: PRIDE project
     2. Name: TgCoq5 proximity proteome in mitochondria and cytosolic fractions using FbxO14 as spatial reference