PXD059826-1

PXD059826 is an original dataset announced via ProteomeXchange.

Title	Temporal wheat proteome remodeling by deoxynivalenol reveals novel detoxification signatures across cultivars
Description	Fusarium head blight is a globally devastating fungal disease resulting in reduced grain yield and quality, along with contamination of grains with dangerous mycotoxins. Consumption of such mycotoxins by humans through processed food or livestock through feed has downstream implications of human and animal health. This interconnectivity across the environment, animal, and human health defines the One Health problem of threatened food safety and security. In this study, we explore remodeling of the wheat proteome upon exposure to a common mycotoxin, deoxynivalenol. We investigate cultivar specific responses to DON exposure in FHB-susceptible (Norwell) and -resistant (Sumai#3) cultivars across a continuum of exposure (i.e., 24 and 120 hours post inoculation), and upon low (i.e., 0.1 mg/mL) and high (1.0 mg/mL) levels of the mycotoxin. This complex experimental design enables us to tease apart the dynamic relationship between cultivar and DON tolerance. Specifically, we define specific proteins and broad categories of remodeling that are common (i.e., reduction in photosynthesis) and unique (i.e., glycosyltransferase) to the cultivars and align with anticipated protective mechanisms. Moreover, we applied an in vitro DON tolerance expression system and determined that induction of ubiquinol oxidase provides heightened protection for yeast growth relative to the negative control, as well as increased protection compared to a well-defined DON detoxifying protein. Our study suggests a new avenue for identification and characterization of novel DON detoxifying proteins as putative biomarkers for selected breeding strategies. Such strategies support the production of wheat varieties with increased tolerance to DON for improved global food safety and security.
HostingRepository	PRIDE
AnnounceDate	2025-06-08
AnnouncementXML	Submission_2025-06-08_09:01:54.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	amidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 09:08:08	ID requested
⏵ 1	2025-06-08 09:01:54	announced

10.1016/J.MCPRO.2025.100988;

submitter keyword: Detoxification, Fusarium graminearum, Proteomics,Wheat, Mycotoxins, Deoxynivalenol

Jennifer Geddes-McAlister
contact affiliation	University of Guelph, Guelph, Ontario, Canada, N1G 2W1
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059826
     1. Label: PRIDE project
     2. Name: Temporal wheat proteome remodeling by deoxynivalenol reveals novel detoxification signatures across cultivars