PXD059814 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Selection of pre-leukemic hematopoietic stem cells driven by distinct extracellular matrix molecules |
| Description | Despite rapid advances in mapping genetic drivers and gene expression changes in hematopoietic stem cells (HSCs), there is a relative paucity of studies at the protein level. Here, we perform a deep, multi-omic characterization (epigenome, transcriptome and proteome) of HSCs carrying a loss-of-function mutation in Tet2, a key driver of increased self-renewal in blood cancers. Using state-of-the-art, multiplexed, low-input mass spectrometry (MS)-based proteomics, we profile wildtype (WT) and TET2-deficient (Tet2-/-) HSCs and show that the proteome captures previously unrecognized molecular processes which define the pre-leukemic HSC molecular landscape. Specifically, we obtain more accurate stratification of WT and Tet2-/- HSCs than transcriptomic approaches and identify extracellular matrix (ECM) molecules as novel points of dysregulation upon TET2 loss. HSC expansion assays using ECM-functionalized hydrogels confirm a selective effect on the expansion of Tet2-mutant HSCs. Taken together, our study represents a comprehensive molecular characterization of Tet2-mutant HSCs and identifies a previously unanticipated role of ECM molecules in regulating self-renewal of disease-driving HSCs. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-13 |
| AnnouncementXML | Submission_2025-11-13_07:03:50.891.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Graeme Benstead-Hume |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-15 05:01:17 | ID requested | |
| ⏵ 1 | 2025-11-13 07:03:51 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: hematopoietic stem cell, extracellular matrix molecules |
Contact List
| Jyoti Choudhary |
|---|
| contact affiliation | Functional Proteomics, ICR |
| contact email | jyoti.choudhary@icr.ac.uk |
| lab head | |
| Graeme Benstead-Hume |
|---|
| contact affiliation | ICR |
| contact email | ghume@icr.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD059814 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059814
- Label: PRIDE project
- Name: Selection of pre-leukemic hematopoietic stem cells driven by distinct extracellular matrix molecules
