PXD059791-1

PXD059791 is an original dataset announced via ProteomeXchange.

Title	Forced Intracellular degradation of Xenoantigens as a modality for cell-based cancer immunotherapy
Description	Given recent leverage of mesenchymal stromal cells (MSCs) as a potent vaccination platform, we investigated whether forced degradation of an expressed experimental antigen fused to small degron sequences could prime potent antitumoral responses. Retrovirally gene-engineered MSCs were evaluated for their in-vitro antigen presentation capacity, nature of generated peptide repertoire and therapeutic potency in syngeneic immunocompetent mice with pre-established solid T-cell lymphoma. Despite lack of noticeable changes in gene expression, MSC-UBvR-OVA vaccination triggered potent T-cell activation which can be attributable to the enriched cell surface presentation of OVA-derived peptides added to elevated mitochondrial ROS production, the latter being associated with efficient antigen processing. Where MSC-UBvR-OVA vaccination successfully controlled tumor growth in cancer-bearing mice, the effect is further enhanced using tranylcypromine-stimulated MSCs and anti-PD-1 combination. Such anti-tumoral response relies on efferocytosis by endogenous phagocytes. Altogether, UBvR facilitated forced antigen degradation represents a plausible modality for future development of tumor antigen-expressing MSC-based vaccine.
HostingRepository	PRIDE
AnnounceDate	2025-01-17
AnnouncementXML	Submission_2025-01-17_04:49:22.624.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059791
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eric Bonneil
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-14 12:08:55	ID requested
⏵ 1	2025-01-17 04:49:22	announced

10.6019/PXD059791;

submitter keyword: xenoantigens,mesenchymal stromal cells, immunopeptidomics, cell-based cancer immunotherapy

Moutih Rafei
contact affiliation	Université de Montréal
contact email	moutih.rafei.1@umontreal.ca
lab head
Eric Bonneil
contact affiliation	Proteomic Platform
contact email	eric.bonneil@umontreal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD059791

PRIDE project URI

• PRIDE
  1. PXD059791
     1. Label: PRIDE project
     2. Name: Forced Intracellular degradation of Xenoantigens as a modality for cell-based cancer immunotherapy