PXD059765-1

PXD059765 is an original dataset announced via ProteomeXchange.

Title	Spatial and development responses in the wheat (Triticum aestivum) leaf highlight the loss of chloroplast protein homeostasis during salt stress.
Description	Salinity stress in wheat affects physiological and biochemical parameters in tissues that alter plant development and ultimately lower crop yield. Shoot tissues are the most sensitive to salinity in wheat plants and accumulate salt over time through the transpiration stream. Rising NaCl concentrations impose physiological responses in leaf tips rather than in leaf bases and align salt effects with the basipetal developmental gradient of the monocot leaf. The role of metabolic processes in generating and responding to this phenotype can be explored by linking distinct changes in ion distributions to those of enzymes from the base to the tip of leaves under salt stress. We confirmed that enzymes for methionine synthesis and lipid degradation pathways increase, concomitantly with proteins in jasmonate synthesis which are key players in plant salt stress-induced responses. Combining the use of Differential Abundance of Protein analysis and Weighted Correlation Network Analysis we have focused on identifying key protein hubs associated with negative salt responses, shedding light on potential sites of salt sensitivity as targets for enhancing salt tolerance in wheat. We found chloroplast protein synthesis machinery, including the 30S and 50S ribosomal proteins, DNA-directed RNA polymerases, and protein synthesis elongation factors, were significantly reduced in abundance and correlated with the altered K+/Na+ ratio along salt-stressed wheat leaves. Additionally, the ATP-dependent caseinolytic protease and filamentous temperature-sensitive H protease, involved in chloroplast protein homeostasis, show decreased abundance with salt. The complex interplay of these processes in and across the leaf affects overall plant viability under salt stress.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:39:49.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bhagya Dissanayake
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-14 01:56:31	ID requested
⏵ 1	2025-05-07 06:39:50	announced

Wijeweera S, Duncan O, Millar AH, Spatial and development responses in the wheat leaf highlight the loss of chloroplast protein homeostasis during salt stress. J Proteomics, 316():105438(2025) [pubmed]

10.1016/j.jprot.2025.105438;

ProteomeXchange project tag: Human Proteome Project

submitter keyword: leaf developmental gradient, protein homeostasis, salinity,wheat

Andrew Harvey Millar
contact affiliation	ARC Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, M316, Perth,WA, 6009, Australia
contact email	harvey.millar@uwa.edu.au
lab head
Bhagya Dissanayake
contact affiliation	University of Western Australia
contact email	bhagya.dissanayake@research.uwa.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059765
     1. Label: PRIDE project
     2. Name: Spatial and development responses in the wheat (Triticum aestivum) leaf highlight the loss of chloroplast protein homeostasis during salt stress.