PXD059757-1

PXD059757 is an original dataset announced via ProteomeXchange.

Title	HIF-1 ATTENUATES THE PROLIFERATIVE EFFECT OF INULIN-DIET THROUGH REWIRING OF METABOLISM
Description	A striking example of the intricate interplay between diet, microbiota, and host is the effect of inulin, a dietary fiber, on the intestinal epithelium. Ingestion of inulin triggers a wide range of epithelial effects in the colon, such as enhanced proliferation, increased production of mucus and antimicrobial peptides, as well as systemic effects on host metabolism and immune function that are dependent on microbiota-derived molecules. In this study, we investigated the impact of inulin on two critical aspects of diet-microbiota-host interactions: intestinal hypoxia and the modulation of hypoxia-inducible factor (HIF)-1 signaling in intestinal epithelial cells (IECs) in mice colon. To achieve this, we employed a multilayered and multi-omics approach, including dietary interventions, in vitro analysis using intestinal organoids, and both genetic and pharmacological interventions. We found that inulin intake enhances intestinal hypoxia, resulting in the stabilization of HIF-1 in IECs, an effect that is both microbiota- and host-dependent. Our study revealed that HIF-1 plays a key role in regulating IEC proliferation and intestinal stem cell (ISC) function. These changes are associated with HIF-1-dependent metabolic alterations in IECs. Our findings uncover a novel mechanism by which HIF-1 acts in the colon: it acts as a molecular brake, modulating cell proliferation in a microbiota-dependent manner and through metabolic reprogramming, highlighting the complexity of the diet-microbiota-host interactions in the gut.
HostingRepository	PRIDE
AnnounceDate	2025-08-23
AnnouncementXML	Submission_2025-08-23_14:30:49.281.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059757
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bradley Smith
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-13 23:08:15	ID requested
⏵ 1	2025-08-23 14:30:49	announced

10.6019/PXD059757;

10.1080/19490976.2025.2543123;

submitter keyword: metabolism, oxidative phosphorylation,hypoxia, dietary fiber, microbiota, intestinal stem cells

Marco Aurelio Remirez Vinolo
contact affiliation	Laboratory of Immunoinflammation, Department of Genetics and Evolution, Microbiology, and Immunology, Institute of Biology, University of Campinas, Brazil
contact email	mvinolo@unicamp.br
lab head
Bradley Smith
contact affiliation	UNICAMP
contact email	a22brad22@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059757
     1. Label: PRIDE project
     2. Name: HIF-1 ATTENUATES THE PROLIFERATIVE EFFECT OF INULIN-DIET THROUGH REWIRING OF METABOLISM