PXD059734-1

PXD059734 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A Multiplexed SEC-MS Approach to Systematically Study the Interplay Between Protein Assembly-States and Phosphorylation Events
Description	A proteins molecular interactions and post-translational modifications (PTMs), such as phosphorylation, can be co-dependent and reciprocally co-regulate each other. Although this interplay is central for many biological processes, a systematic method to simultaneously study assembly-states and PTMs from the same sample is critically missing. Here, we introduce SEC-MX (Size Exclusion Chromatography fractions MultipleXed), a global quantitative method combining Size Exclusion Chromatography and PTM-enrichment for simultaneous characterization of PTMs and assembly-states. SEC-MX enhances throughput, allows phosphopeptide enrichment, and facilitates quantitative differential comparisons between biological conditions. Applying SEC-MX to HEK293 and HCT116 cells, we generated a proof-of-concept dataset mapping thousands of phosphopeptides and their assembly-states. Our analysis revealed intricate relationships between phosphorylation events and assembly-states and generated testable hypotheses for follow-up studies. Overall, we establish SEC-MX as a valuable tool for exploring protein functions and regulation beyond abundance changes.
HostingRepository	MassIVE
AnnounceDate	2025-01-13
AnnouncementXML	Submission_2025-01-13_09:28:58.010.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Jovanovic
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-13 09:27:16	ID requested
⏵ 1	2025-01-13 09:28:58	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Protein assembly-states, PTM, Size Exclusion chromatography, protein interactions, phosphorylation

submitter keyword: Protein assembly-states, PTM, Size Exclusion chromatography, protein interactions, phosphorylation

Contact List

Marko Jovanovic
contact affiliation	Columbia University
contact email	mj2794@columbia.edu
lab head
Marko Jovanovic
contact affiliation	Columbia
contact email	mj2794@columbia.edu
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000096001/