PXD059719-1

PXD059719 is an original dataset announced via ProteomeXchange.

Title	Identification of celiac-disease active peptides in gluten-free beers by nanoLC-MS/MS
Description	Barley beers may be rendered gluten-free by specialized brewing processes. One controversial issue is whether these beers contain residual gluten peptides carrying at least one celiac disease (CeD)-active epitope, because routine methods to assess whether the beer is gluten-free like competitive enzyme-linked immunosorbent assays (ELISA) may fail to detect potentially harmful peptides. We used untargeted nano-liquid chromatography-tandem mass spectrometry (nanoLC MS/MS) to identify CeD-active peptides in 21 gluten-free barley beers and four regular or carbohydrate-reduced beers. While the G12 ELISA confirmed gluten levels below 20 mg/kg in all gluten-free beers, the R5 ELISA detected gluten levels in four beers exceeding this threshold, highlighting method discrepancies. nanoLC-MS/MS identified 44 CeD active peptides in seven gluten-free and four non-gluten-free beers, of which 17 CeD-active peptides had no ELISA epitope and therefore escape detection. Accurate quantitation of these peptides combined with in vivo toxicity assessment will be needed to provide guidance on clinical relevance.
HostingRepository	PRIDE
AnnounceDate	2026-04-01
AnnouncementXML	Submission_2026-04-01_11:18:21.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eleonora Tissen
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: NEWT:4513; scientific name: Triticum aestivum (Wheat); NCBI TaxID: NEWT:4565;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-13 02:50:02	ID requested
⏵ 1	2026-04-01 11:18:21	announced

10.1016/J.AFRES.2026.101952;

submitter keyword: None

Katharina Scherf
contact affiliation	Leibniz Institute for Food Systems Biology at the Technical University of Munich
contact email	k.scherf.leibniz-lsb@tum.de
lab head
Eleonora Tissen
contact affiliation	Leibniz Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany
contact email	e.tissen.leibniz-lsb@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD059719

PRIDE project URI

• PRIDE
  1. PXD059719
     1. Label: PRIDE project
     2. Name: Identification of celiac-disease active peptides in gluten-free beers by nanoLC-MS/MS