⮝ Full datasets listing
PXD059705-1
PXD059705 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | FeaSion Uncovers the Multifaceted Co-transcriptional Roles of RNAPII CTD Phosphorylation Regulated by Cancer Hallmark Genes |
| Description | The phosphorylation of RNAPII's C-terminal domain (CTD) has been extensively studied, yet functional investigations into all five known phosphorylation states (pY1, pS2, pT4, pS5, pS7) simultaneously are scarce, and the number of identified regulators is limited. Utilizing the FeaSion (Feature-Screening-Function) strategy, we characterized RNAPII CTD phosphorylation by identifying 706 interactors across these five states. Additionally, we conducted a CRISPR-FACS screen, revealing 2,067 potential regulators, which include 79 cancer hallmark genes, 94 kinases, and 21 phosphatases. Our study demonstrates that mutations at these phosphorylation sites broadly influence various transcriptional processes and preferentially alter co-transcriptional events, including histone modifications (H3K36me3, H2AZ, H2AZ.ac) and RNA splicing. This work offers a comprehensive perspective on RNAPII CTD phosphorylation and its regulatory network, presenting a valuable resource for future research in developmental biology and disease mechanisms. |
| HostingRepository | iProX |
| AnnounceDate | 2025-01-10 |
| AnnouncementXML | Submission_2025-10-27_17:35:47.043.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lijun Bao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-12 21:36:28 | ID requested | |
| ⏵ 1 | 2025-10-27 17:35:47 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: RNAPII, CTD phosphorylation |
Contact List
| Xiong Ji | |
|---|---|
| contact affiliation | Peking University |
| contact email | xiongji@pku.edu.cn |
| lab head | |
| Lijun Bao | |
| contact affiliation | Peking University |
| contact email | baolijun0906@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
