PXD059691-1

PXD059691 is an original dataset announced via ProteomeXchange.

Title	Utilizing a Negative Enrichment Strategy to Profile Protein Methylation, Leveraging the Orthogonality of LysargiNase and Trypsin-1
Description	Protein methylation, a prevalent post-translational modification, plays crucial roles in chromatin remodeling and gene transcription. A deeper understanding protein methylation in these biological processes necessitates a comprehensive characterization of the methylation sites. However, the methylation group induces minimal changes in the size and electrostatic status of lysine/arginine residues. This significantly increases the difficulty of distinguishing the methylation sites from the non-methylation sites. In this study, we developed a strategy to enrich protein methylation, termed the Negative Enrichment Strategy for Profiling Protein Methylation, to comprehensively analyze lysine/arginine methylation. Initially, proteins were digested using LysargNase to generate peptides containing methylated or non-methylated lysine/arginine at the Nterminus. Subsequently, the N-terminal free α-amines of the LysargiNase-generated peptides were selectively blocked using formaldehyde in an acidic solution. As t
HostingRepository	iProX
AnnounceDate	2025-01-08
AnnouncementXML	Submission_2025-01-12_19:14:15.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuxian Wei
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	methylthiolated residue; dimethylated residue; trimethylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-12 18:55:06	ID requested
⏵ 1	2025-01-12 19:14:15	announced

Dataset with its publication pending

submitter keyword: Negative Enrichment Strategy, Protein Methylation

Bo Jiang
contact affiliation	Dalian Institute of Chemical Physics
contact email	jiangbo@dicp.ac.cn
lab head
Shuxian Wei
contact affiliation	Dalian Institute of Chemical Physics
contact email	weishuxian@dicp.ac.cn
dataset submitter

iProX dataset URI