PXD059671-1

PXD059671 is an original dataset announced via ProteomeXchange.

Title	Diversity and Heterogeneity of Extracellular Vesicles and Brain-Penetrating Amembranous Non-Vesicular Nanoparticles in Glioblastoma
Description	Advances in understanding intercellular communication through extracellular vesicles (EVs) and non-vesicular extracellular nanoparticles (NVEPs) have been limited by their cellular heterogeneity and unclear origins. Using sequential ultracentrifugation, we identified high- and low-density EVs and performed multi-omics analyses on four EV/NVEP categories, including supermeres. Our findings reveal that extracellular RNA, RNA-binding proteins, and other cellular proteins are differentially expressed among EVs and NVEPs. Notably, supermeres can penetrate the blood-brain barrier, in a manner dependent on their intact RNA and protein structure, and distribute throughout the CNS. Supermeres preferentially interact with microglia at higher levels than other CNS cell types. These interactions lead to decreased TGFβ expression, suggesting an immunomodulatory role. This study highlights supermeres as a promising platform for CNS therapeutic delivery.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_17:13:50.955.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-11 12:32:43	ID requested
⏵ 1	2025-10-12 17:13:52	announced

10.1002/jev2.70168;

Xu T, Paulo JA, Zhang P, Liu X, Nguyen A, Cheng Y, Massick C, Zhang Y, Jeppesen DK, Zhang Q, Higginbotham JN, Tutanov OS, Krichevsky AM, Chiu DT, Gygi SP, Vickers KC, Franklin JL, Coffey RJ, Charest A, Heterogeneity of Extracellular Vesicles and Non-Vesicular Nanoparticles in Glioblastoma. J Extracell Vesicles, 14(10):e70168(2025) [pubmed]

submitter keyword: supermeres, extracellular nanoparticles, exomeres, extracellular vesicles,Glioblastoma, EGFR

Alain Charest
contact affiliation	Department of Medicine Beth Israel Deaconess Medical Center Boston, MA
contact email	acharest@bidmc.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD059671

PRIDE project URI

• PRIDE
  1. PXD059671
     1. Label: PRIDE project
     2. Name: Diversity and Heterogeneity of Extracellular Vesicles and Brain-Penetrating Amembranous Non-Vesicular Nanoparticles in Glioblastoma