PXD059664-1

PXD059664 is an original dataset announced via ProteomeXchange.

Title	Characterization and chemoproteomic profiling of protein O-GlcNAcylation in SOD1-G93A mouse model
Description	ALS is a devastating motor neuron disease. Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification has been found to affect the processing of several important proteins implicated in ALS. However, the O-GlcNAcylated proteome remains unexplored during ALS progression. Herein, we probed the dynamic changes of O-GlcNAcylation in SOD-G93A mice, the most widely used animal model for studying ALS pathogenesis. We discovered that the overall O-GlcNAc level is significantly decreased at the disease end stage. Correlatively, a great increase of OGA was observed. By using isoPTOP, a recently reported chemoproteomics strategy, we furthermore reported the identification of 568 high-confidence O-GlcNAc sites from end-stage SOD1-G93A and NTG mice. Of the 568 sites, 226—many of which occurred on neuronal function and structure-related proteins—were found to be dynamically regulated. These results provide a valuable resource for dissecting the functional role of O-GlcNAcylation in ALS and shed light on promising therapeutic avenues for ALS.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:27:34.262.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Hao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-10 19:31:35	ID requested
⏵ 1	2025-05-07 06:27:35	announced

Hao Y, Li Z, Du X, Xie Q, Li D, Lei S, Guo Y, Characterization and chemoproteomic profiling of protein O-GlcNAcylation in SOD1-G93A mouse model. Mol Med, 31(1):82(2025) [pubmed]

10.1186/s10020-025-01134-4;

submitter keyword: Amyotrophic lateral sclerosis (ALS), SOD1-G93A mice, O-linked β-N-acetylglucosamine (O-GlcNAc), LC-MS/MS

Yansu Guo
contact affiliation	Beijing Geriatric Healthcare and Disease Prevention Center, Xuanwu Hospital, Capital Medical University, Beijing, China.
contact email	gys188@163.com
lab head
Yi Hao
contact affiliation	Shandong University
contact email	yhao328@pku.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059664
     1. Label: PRIDE project
     2. Name: Characterization and chemoproteomic profiling of protein O-GlcNAcylation in SOD1-G93A mouse model