PXD059663 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ascorbate peroxidase (APEX2) -mediated proximity-based proteomics for HTT protein interactions |
| Description | Huntington’s Disease (HD), a progressive neurodegenerative disorder with no disease-modifying therapies, is caused by a CAG repeat expansion in the HD gene encoding polyglutamine-expanded huntingtin (HTT) protein. Mechanisms of HD cellular pathogenesis and cellular functions of the normal and mutant HTT proteins are still not completely understood. HTT protein has numerous interaction partners, and it likely provides a scaffold for assembly of multiprotein complexes many of which may be altered in HD. Previous studies have implicated DNA damage response in HD pathogenesis. Gene transcription and RNA processing has also emerged as molecular mechanisms associated with HD. Here we used multiple approaches to identify HTT interactors in the context of DNA damage stress. We present evidence for the role for HTT in double strand break (DSB) repair mechanism. Our results indicate that HTT interacts with many proteins involved in the regulation of interconnected DNA repair/remodeling and RNA processing pathways. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-16 |
| AnnouncementXML | Submission_2026-03-16_09:37:38.032.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD059663 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Tamara Ratovitski |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-10 15:18:08 | ID requested | |
| ⏵ 1 | 2026-03-16 09:37:38 | announced | |
Publication List
Keyword List
| submitter keyword: Huntington’s Disease, protein interactions, huntingtin, DNA damage, neurodegeneration |
Contact List
| Christopher A. Ross |
|---|
| contact affiliation | Division of Neurobiology, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD |
| contact email | caross@jhu.edu |
| lab head | |
| Tamara Ratovitski |
|---|
| contact affiliation | Division of Neurobiology, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore MD |
| contact email | tratovi1@jhmi.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD059663 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059663
- Label: PRIDE project
- Name: Ascorbate peroxidase (APEX2) -mediated proximity-based proteomics for HTT protein interactions
