PXD059662-1

PXD059662 is an original dataset announced via ProteomeXchange.

Title	The brain interactome of a permissive prion replication substrate
Description	Bank voles are susceptible to prion strains from many different species, yet the molecular mechanisms underlying the ability of bank vole prion protein (BVPrP) to function as a universal prion acceptor remain unclear. Potential differences in molecular environments and protein interaction networks on the cell surface of brain cells may contribute to BVPrP’s unusual behavior. To test this hypothesis, we generated knock-in mice that express physiological levels of BVPrP (M109 isoform) and employed mass spectrometry to compare the interactomes of mouse (Mo) PrP and BVPrP following mild in vivo crosslinking of brain tissue. Substantial overlap was observed between the top interactors for BVPrP and MoPrP, with established PrP-interactors such as neural cell adhesion molecules, subunits of Na+/K+-ATPases, and contactin-1 being equally present in the two interactomes. We conclude that the molecular environments of BVPrP and MoPrP in the brains of mice are very similar. This suggests that the unorthodox properties of BVPrP are unlikely to be mediated by differential interactions with other proteins.
HostingRepository	PRIDE
AnnounceDate	2026-07-06
AnnouncementXML	Submission_2026-07-06_15:11:22.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerold Schmitt-Ulms
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-10 14:26:37	ID requested
⏵ 1	2026-07-06 15:11:23	announced

10.1016/J.NBD.2025.106802;

submitter keyword: mass spectrometry, bank voles,prion, knock-in mice, protein-protein interactions

Joel Watts amd Gerold Schmitt-Ulms
contact affiliation	Biochemistry
contact email	joel.watts@utoronto.ca
lab head
Gerold Schmitt-Ulms
contact affiliation	University of Toronto
contact email	g.schmittulms@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD059662

PRIDE project URI

• PRIDE
  1. PXD059662
     1. Label: PRIDE project
     2. Name: The brain interactome of a permissive prion replication substrate