PXD059655-1

PXD059655 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals the regulation of lentivirus-mediated THBS1 overexpression in ALG1 and its mechanism in meibomian gland carcinoma
Description	Meibomian gland carcinoma (MGC) is an aggressive and poorly prognostic eyelid malignancy. Previous studies have demonstrated that miR-3907 is highly expressed in MGC, accelerating tumor progression by negatively regulating THBS1. This study delves into the downstream regulatory mechanisms of THBS1. Using 4D-label-free proteomics, ALG1 was identified as the downstream protein with the highest differential expression and consistent sequencing results following lentivirus-mediated THBS1 overexpression. First, protein-protein docking models predicted that THBS1 interacted with ALG1 through hydrogen bonds formed by key amino acid residues such as ASN-991 and MET-58, creating a stable docking structure. Immunofluorescence and co-immunoprecipitation assays further confirmed the co-localization and interaction between THBS1 and ALG1. In MGC cells, the mRNA expression of ALG1 was notably higher than that in normal meibomian gland cells. Functional assays demonstrated that knockdown of ALG1 evidently inhibited cell malignant properties. Additionally, ALG1 knockdown modulated the expression of epithelial-mesenchymal transition-related genes, which are key modulators of tumor metastasis. Conversely, ALG1 overexpression produced the opposite effects. Therefore, ALG1, as part of the miR-3907/THBS1/ALG1 axis, can serve as a biomarker for targeted diagnosis and treatment of MGC. This study provides valuable insights into potential targeted therapies and personalized treatment strategies for MGC.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:05:37.996.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-10 12:38:03	ID requested
⏵ 1	2025-09-28 16:05:38	announced

10.3892/ol.2025.15263;

Wang W, Wang H, Wu G, Liu X, Zhu L, Tian F, Lin T, Proteomics reveals the regulation of ALG1 expression by lentivirus-mediated THBS1 overexpression and its mechanism in meibomian gland carcinoma. Oncol Lett, 30(5):517(2025) [pubmed]

submitter keyword: 4D-label-free proteomics Meibomian gland carcinoma Thrombospondin-1 Chitobiosyldiphosphodolichol beta-mannosyltransferase

wei wang
contact affiliation	Tianjin Medical University
contact email	1109418072@qq.com
lab head
Wei Wang
contact affiliation	Tianjin Medical University Eye Hospital
contact email	1109418072@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059655
     1. Label: PRIDE project
     2. Name: Proteomics reveals the regulation of lentivirus-mediated THBS1 overexpression in ALG1 and its mechanism in meibomian gland carcinoma