PXD059637 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DNGR-1/CLEC9A signaling limits cDC1 activation to optimize crosspresentation of dead cell antigens |
| Description | Innate immune receptors induce activation of conventional dendritic cells (cDCs) and enhance antigen (cross-)presentation, favoring immune responses. DNGR-1 (CLEC9A), a receptor expressed by type 1 cDCs (cDC1s) and implicated in immunity to viruses and cancers, recognizes F-actin exposed on dead cell remnants and promotes cross-presentation of associated antigens. Here, we show that DNGR-1 does not trigger cDC1 activation, in part by recruiting phosphatase SHIP1, a process governed by a single amino acid adjacent to the signaling motif of the receptor. Substituting this residue converts DNGR-1 into an activating receptor but decreases its induction of cross-presentation of dead cell-associated antigens. Interestingly, introducing the reverse mutation into related receptor Dectin-1 impairs its activation capacity while enhancing its ability to promote cross-presentation. These findings reveal an unexpected functional trade-off in receptor signaling and suggest that DNGR-1 has evolved to prioritize antigen cross-presentation over cellular activation, possibly to minimize inflammatory responses to dead cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-28 |
| AnnouncementXML | Submission_2025-10-28_07:23:22.011.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mark Skehel |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-10 07:37:51 | ID requested | |
| ⏵ 1 | 2025-10-28 07:23:22 | announced | |
Publication List
Keyword List
| submitter keyword: Mouse, dendritic cell, lc-ms/ms, innate immune receptor |
Contact List
| Caetano Reis e Sousa |
|---|
| contact affiliation | Immunobiology Laboratory, The Francis Crick Institute, London, UK |
| contact email | caetano@crick.ac.uk |
| lab head | |
| Mark Skehel |
|---|
| contact affiliation | Francis Crick Institute |
| contact email | skehelm@crick.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059637
- Label: PRIDE project
- Name: DNGR-1/CLEC9A signaling limits cDC1 activation to optimize crosspresentation of dead cell antigens
