PXD059579-1

PXD059579 is an original dataset announced via ProteomeXchange.

Title	MS-based proteomic characterization of Toxoplasma gondii GSK3 proxisome
Description	In an effort to identify novel drug candidates against T. gondii parasites, an interesting compound was identified, allowing to inhibit tachyzoite growth without impacting the HFF monolayer. A forward genetic screen identifies TgGSK3 as the enzyme targeted by this molecule. To investigate the biological function of TgGSK3 and its regulatory mechanisms, we performed in vivo biotinylation using the proximity labeling approach employing BioID2 with TgGSK3 as the bait protein. To identify candidate interacting partners or substrates of TgGSK3, biotinylated proteins were enriched from lysates using streptavidin affinity purification. As a control, we conducted a mock experiment with parasites that do not express BioID2 fusion protein (RH ku80).
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-15_10:32:18.956.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059579
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-09 02:16:01	ID requested
⏵ 1	2025-09-15 10:32:19	announced

10.6019/PXD059579;

submitter keyword: GSK3,Apicomplexa, proximity labeling, Toxoplasma gondii, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059579
     1. Label: PRIDE project
     2. Name: MS-based proteomic characterization of Toxoplasma gondii GSK3 proxisome