PXD059557-1

PXD059557 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	From dimer to tetramer: the evolutionary trajectory of C4 photosynthetic-NADP-ME oligomeric state in Poaceae
Description	In maize, two plastidic NADP-malic enzyme (C4-NADP-ME) isoforms are present: C4-NADP-ME and nonC4-NADP-ME, each with distinct kinetic and structural features. To investigate the differences in oligomeric states of C4- and nonC4-NADP-ME in plant tissues, we analyzed extracts of maize from etiolated leaves and roots using native PAGE coupled with an in-gel NADP-ME activity assay. All protein bands exhibiting NADP-ME activity were excised and analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:22:37.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: NEWT:4577;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-08 10:07:26	ID requested
⏵ 1	2026-05-24 16:22:38	announced

Publication List

10.1093/molbev/msag056;
B, ö, hm JM, Willms S, Ferrao O, Buitrago-Arango M, H, ü, dig M, Poschmann G, Fazelnia N, Nagel-Steger L, Klinke S, Drakonaki A, Gatsogiannis C, Tronconi MA, Alvarez CE, Maurino VG, A milestone in C4 carbon concentration mechanism evolution: structural remodeling of NADP-malic enzyme in Poaceae. Mol Biol Evol, 43(4):(2026) [pubmed]

10.1093/molbev/msag056;

B, ö, hm JM, Willms S, Ferrao O, Buitrago-Arango M, H, ü, dig M, Poschmann G, Fazelnia N, Nagel-Steger L, Klinke S, Drakonaki A, Gatsogiannis C, Tronconi MA, Alvarez CE, Maurino VG, A milestone in C4 carbon concentration mechanism evolution: structural remodeling of NADP-malic enzyme in Poaceae. Mol Biol Evol, 43(4):(2026) [pubmed]

Keyword List

submitter keyword: Zea mays, C4-NADP-ME, native PAGE, oligomeric state, C4 photosynthetic pathway

submitter keyword: Zea mays, C4-NADP-ME, native PAGE, oligomeric state, C4 photosynthetic pathway

Contact List

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine Heinrich Heine University Düsseldorf Universitätsstraße 1 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD059557

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Repository Record List

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