PXD059541 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome analysis of biofilm extracellular vesicles produced by Granulicatella adiacens and Granulicatella elegans. |
| Description | Granulicatella adiacens and Granulicatella elegans are non-motile, non-spore-forming, facultatively anaerobic Gram-positive cocci. They are part of the normal oral flora but causes serious infections such as infective endocarditis. When oral bacteria accidentally enter the bloodstream due to transient tissue damage during dental procedures, they have the potential to attach to the endocardium or an equivalent surface of an indwelling prosthesis and cause infection. Many bacterial species produce extracellular vesicles (EVs) as a virulence strategy. The study of these EVs was initially limited to Gram-negative bacteria; however, their production was also observed in some Gram-positive bacteria. Recent research in our lab has found that G. adiacens produces abundant EVs in planktonic life style. However, EVs production by G. adiacens in biofilm mode is unknown. Therefore, the objective is to isolate and characterize EVs produced by this bacterium in biofilm mode. G. adiacens CCUG 27809 will be cultured on chocolate blood agar with 0.001% pyridoxal hydrochloride at 37 °C and 5% CO2 for 2 days. The EVs will be isolated using differential centrifugation and filtration protocol and then observed using electron scanning microscopy. Proteins in the biofilm EVs will be identified using mass spectrometry. Unraveling the EVs proteome composition of G. adiacens biofilms may help elucidate intricate virulence strategies of this species. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-05 |
| AnnouncementXML | Submission_2026-01-04_16:46:50.837.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Maribasappa Karched |
| SpeciesList | scientific name: Granulicatella elegans; NCBI TaxID: NEWT:137732; scientific name: Granulicatella adiacens; NCBI TaxID: NEWT:46124; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-08 02:50:00 | ID requested | |
| ⏵ 1 | 2026-01-04 16:46:51 | announced | |
Publication List
Keyword List
| submitter keyword: Granulicatella, Biofilms, Infective endocarditis, Proteomics, Extracellular vesicles |
Contact List
| Maribasappa Karched, PhD |
|---|
| contact affiliation | Department of Bioclinical Sciences College of Dentistry Kuwait University Kuwait. |
| contact email | maribasappa.karched@gmail.com |
| lab head | |
| Maribasappa Karched |
|---|
| contact affiliation | Faculty of Dentistry
Kuwait University |
| contact email | maribasappa.karched@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD059541 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059541
- Label: PRIDE project
- Name: Proteome analysis of biofilm extracellular vesicles produced by Granulicatella adiacens and Granulicatella elegans.
