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PXD059520-1

PXD059520 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRetina-Targeted 17β-Estradiol by the DHED Prodrug Rescues Visual Function and Actuates Neuroprotective Protein Net-works after Optic Nerve Crush in a Rat Model of Surgical Menopause. Katalin Prokai-Tatrai, Khadiza Zaman, Ammar Kapic, Kelleigh Hogan , Gabriela Sanchez-Rodriguez, Anna E. Silverio Vien Nguyen , Laszlo Prokai and Andrew J. Feola
DescriptionThe association between 17β-estradiol (E2) deprivation, seen in menopause and a risk for devel-oping glaucoma, has been shown. Thus, exogenous supplementation of E2 may protect against retinal ganglion cell (RGC) degradation and vision loss. Here, we investigated the utility of topical 10β,17β-dihydroxyestra-1,4-dien-3-one (DHED), a prodrug of E2 that selectively produces the neuroprotective hormone in the retina, on visual function outcomes after optic nerve crush (ONC) and ovariectomy (OVX). We used female Brown Norway rats that underwent either Sham or OVX surgeries. After ONC, OVX animals received DHED eye drops for 12 weeks. Visual function, via the optomotor reflex, and retinal thickness, via optical coherence tomography, were followed longitudinally. Afterward, we performed mass spectrometry-based label-free retina proteomics to survey retinal protein interaction networks in our selected animal model and to identify E2-responsive proteins after OVX on neurodegeneration. We found that ONC with OVX caused a significant decline in visual functions that were ameliorated by DHED treatments. Discov-ery-driven retina proteomics identified numerous proteins associated with neurodegenerative processes due to ONC that were remediated by DHED eye drops. Altogether, our three-pronged phenotypic preclinical evaluation of the topical DHED in the OVX + ONC model of glaucoma re-veals the therapeutic potential of the prodrug to prevent visual deficits after glaucomatous retinal injury.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_06:30:42.909.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD059520
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLaszlo Prokai
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-07 13:22:43ID requested
12025-05-07 06:30:43announced
Publication List
Prokai-Tatrai K, Zaman K, Kapic A, Hogan K, Sanchez-Rodriguez G, Silverio AE, Nguyen V, Prokai L, Feola AJ, -Estradiol by the DHED Prodrug Rescues Visual Function and Actuates Neuroprotective Protein Networks After Optic Nerve Crush in a Rat Model of Surgical Menopause. Int J Mol Sci, 26(5):(2025) [pubmed]
10.6019/PXD059520;
10.3390/ijms26051846;
Keyword List
submitter keyword: bioinformatics
DHED
estrogen
glaucoma
menopause
optic nerve crush
optomotor response
protein–protein interaction networks
quantitative label-free retina proteomics
Contact List
Laszlo Prokai, Ph.D., D.Sc
contact affiliationProfessor and Robert A. Welch Chair in Biochemistry Department of Pharmacology and Neuroscience University of North Texas Health Science Center at FortWorth
contact emaillaszlo.prokai@unthsc.edu
lab head
Laszlo Prokai
contact affiliationDepartment of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact emailadvancedms@unthsc.edu
dataset submitter
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Dataset FTP location
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