PXD059509-1

PXD059509 is an original dataset announced via ProteomeXchange.

Title	Cryo-EM structure of photosystem II supercomplex from Chlorella ohadii, a green microalga with extreme phototolerance
Description	Photosystem II (PSII) is essential for energy conversion during oxygenic photosynthesis in plants and algae. Chlorella ohadii, one of the fastest multiplying green alga, thrives under the harsh desert sun but lacks the standard PSII photoprotective mechanisms involving LhcSR/PsbS proteins or protein phosphorylation. Here, we present analysis of cryo-EM structure of the PSII supercomplex from C. ohadii at 2.9Å resolution to determine whether the exceptional resistance to desert conditions has a structural basis in PSII. The structure reveals a novel PsbO isoform and additional subunits, PsbR and PsbY, which enhance core complex stability through extensive interactions. Furthermore, the trimeric light-harvesting complexes (LHCII) are bound to the PSII core by specific light-harvesting proteins whose down-regulation in response to high light conditions implies a reduction in the number of bound LHCII trimers. These modifications play a crucial role in maintaining PSII stability and photoprotection, allowing C. ohadii to survive in extreme conditions.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_01:32:08.813.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Chlorella ohadii; NCBI TaxID: NEWT:2649997;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-07 07:01:25	ID requested
⏵ 1	2025-10-06 01:32:09	announced

10.1038/S41477-023-01483-0;

submitter keyword: proteomics, thylakoid membranes,Chlorella ohadii, PSII, photosystem, timsTOF Pro

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059509
     1. Label: PRIDE project
     2. Name: Cryo-EM structure of photosystem II supercomplex from Chlorella ohadii, a green microalga with extreme phototolerance