PXD059503 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Combined cysteine oxidation (C6-CPT/CysPAT) proteomics and phosphoproteomics of pervanadate-treated of HEK293T cells |
| Description | HEK293T cells were treated with pervanadate for 0, 5 or 15 minutes to evaluate the effects on the phosphorylation and cysteine oxidation status across the proteome. Samples were labelled withTMT 18plex and C6-CPT/CysPAT labelling was used to allow the simultaneous enrichment of both the phosphoproteome and total vs. oxidised cysteine proteome. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-22 |
| AnnouncementXML | Submission_2026-06-21_17:15:26.031.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Harvey Johnston |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | dihydroxylated residue; N-ethylmaleimide derivatized cysteine; L-cysteic acid (L-cysteine sulfonic acid) |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-07 05:05:25 | ID requested | |
| ⏵ 1 | 2026-06-21 17:15:26 | announced | |
Publication List
| Mulholland KE, Bourguet M, Cheng N, Rahman O, Ezeri, ņ, a D, Daly LA, Lai T, Aldaz Casanova S, Featherston T, Creixell P, Eyers CE, Messens J, Eyers PA, Byrne DP, Sharpe HJ, Pervanadate-induced oxidation relieves autoinhibition of the protein tyrosine kinase SRC. Sci Signal, 19(921):eady9437(2026) [pubmed] |
| 10.1126/scisignal.ady9437; |
Keyword List
| submitter keyword: HEK293T, cysteine, C6-CysPAT, C6-CPT, redox, phosphoproteomics, pervanadate |
Contact List
| Hayley Sharpe |
|---|
| contact affiliation | Hayley Sharpe, Signalling, Babraham Institute, Cambridge, UK |
| contact email | Hayley.Sharpe@babraham.ac.uk |
| lab head | |
| Harvey Johnston |
|---|
| contact affiliation | The Babraham Institute |
| contact email | harvey.johnston@babraham.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD059503 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059503
- Label: PRIDE project
- Name: Combined cysteine oxidation (C6-CPT/CysPAT) proteomics and phosphoproteomics of pervanadate-treated of HEK293T cells
