PXD059501-1

PXD059501 is an original dataset announced via ProteomeXchange.

Title	In vitro Protein Expression Profile of Cultivated Muscle Cells from Labeo rohita
Description	A quantitative proteomics approach was used to investigate in Labeo rohita cell line across three passages P1, P15 and P25. A new cell line developed from the muscle tissue of L. rohita (LR) was utilized to understand the proteome in-depth. Comparative proteome analysis was performed for LR muscle cells at different passages (1, 15 and 25) using label free quantitative proteomics. High-resolution mass spectrometry was used for data using label-free quantification. Data were further analysed using a statistical tool across passages of rohu muscle cell line. Functional analysis of differentially expressed proteins was marked as glycolytic (eno1 and eno3), metabolic (pgm, smyd1, ak1, aldob and bpgm), cytoplasmic ribosomal (rpl27, rpl3 and eifa5) and carbon metabolic (pdh) pathways across the three passages. The proteins observed across the pathways were also correlated with the differentiation of LR muscle cells during myogenesis. The current study provides insight to determine the different groups of proteins that are expressed during the development of myotubes.
HostingRepository	PRIDE
AnnounceDate	2025-02-19
AnnouncementXML	Submission_2025-02-19_02:58:16.968.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059501
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nevil Pinto
SpeciesList	scientific name: Labeo rohita (Indian major carp); NCBI TaxID: 84645;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-07 04:45:48	ID requested
⏵ 1	2025-02-19 02:58:17	announced

10.1038/S41598-025-88900-W;

10.6019/PXD059501;

ProteomeXchange project tag: Skeletal Muscle Proteome (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP)

submitter keyword: Cell culture,Labeo rohita, myogenesis, LC-MS/MS

Nevil Pinto
contact affiliation	ICAR-CIFE, Mumbai-400061
contact email	nevilpinto1@gmail.com
lab head
Nevil Pinto
contact affiliation	ICAR-CIFE, Mumbai
contact email	nevilpinto1@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059501
     1. Label: PRIDE project
     2. Name: In vitro Protein Expression Profile of Cultivated Muscle Cells from Labeo rohita