PXD059433-1

PXD059433 is an original dataset announced via ProteomeXchange.

Title	A comparative proteomic analysis of resistant and susceptible stylo against C. gloeosporioides reveals that SgATL31 is involved in disease resistance by promoting ROS burst
Description	Stylo (Stylosanthes spp.) is a dominant leguminous forage, widely cultivated in tropical and subtropical areas. Anthracnose caused by Colletotrichum gloeosporioides is one of the most destructive diseases that limit the yield of stylo. Therefore, screening resistance varieties and improving the resistance of stylo are crucial strategies to control stylo anthracnose. In this study, the resistance evaluation of main variety RY2 and 39 stylo germplasms against C. gloeosporioides was performed, 12 stylo germplasms were rated as highly resistant (HR). Among them, 2001-84 was considered to be the most disease-resistant germplasm. Then, the stylo phenotype and the infection process of C. gloeosporioides revealed that the disease severity in resistant germplasm 2001-84 was significantly milder than susceptible germplasm RY2, and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR) and total antioxidant capacity (T-AOC) were higher than those in RY2 at different time post inoculation. Phosphoproteomics revealed that differentially phosphoproteins between 2001-84 and RY2 were significantly enriched in oxidoreductase activity at 96 hpi. An E3 ubiquitin ligase, SgATL31, were identified in both phosphoproteomics and plasma membrane proteomics, and overexpression of SgATL31 enhanced resistance to C. gloeosporioides in Arabidopsis. Additionally, SgATL31 promoted the accumulation of ROS in Arabidopsis leaves and stylo protoplasts under chitin treatment. Overall, this study identified the disease-resistant germplasm 2001-84 and elucidated that SgATL31 enhances resistance to anthracnose in Arabidopsis by inducing the accumulation of ROS. This study provided a reference for further exploring the functions of SgATL31 in C. gloeosporioides infection.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_17:23:23.038.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	丽云 杨
SpeciesList	scientific name: Stylosanthes guianensis subsp. guianensis; NCBI TaxID: 198726;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-04 00:39:34	ID requested
⏵ 1	2025-07-13 17:23:23	announced

Yang L, Long Y, Gao M, Zhang S, Gao J, Luo L, Jiang L, E3-Ubiquitin Ligase SgATL31 Promotes Anthracnose Resistance in Stylosanthes by Modulating ROS Burst and Antioxidant Defence: A Proteomic and Functional Study. Mol Plant Pathol, 26(7):e70122(2025) [pubmed]

10.1111/mpp.70122;

submitter keyword: Colletotrichum gloeosporioides, plasma membrane proteomics, evaluation of disease-resistant resources, SgATL31, phosphoproteomics,Stylosanthes

Liyun Yang
contact affiliation	School of Tropical Agriculture and Forestry, Hainan University
contact email	1058200235@qq.com
lab head
丽云 杨
contact affiliation	海南大学
contact email	1058200235@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059433
     1. Label: PRIDE project
     2. Name: A comparative proteomic analysis of resistant and susceptible stylo against C. gloeosporioides reveals that SgATL31 is involved in disease resistance by promoting ROS burst