PXD059407-1

PXD059407 is an original dataset announced via ProteomeXchange.

Title	MDA-MB-231 blebbisome proteomics
Description	Cells secrete a large variety of extracellular vesicles (EVs) to engage in cell-to-cell and cell-to-environment intercellular communication. EVs are functionally involved in many physiological and pathological processes by interacting with cells that facilitate transfer of proteins, lipids and genetic information. However, our knowledge of EVs is incomplete. We show that cells actively release exceptionally large (up to 20 µm) membrane-enclosed vesicles that exhibit active blebbing behavior, and we therefore have termed them blebbisomes. Blebbisomes contain an array of cellular organelles that include functional mitochondria and multivesicular endosomes yet lack a definable nucleus. We provide proteomic analysis of blebbisomes, large and small EVs released from metastatic MDA-MB-231 breast cancer cells. Blebbisomes are released from normal and cancer cells, can be observed by direct imaging of cancer cells in vivo, and are present in normal bone marrow.
HostingRepository	PRIDE
AnnounceDate	2025-01-05
AnnouncementXML	Submission_2025-01-04_19:22:02.700.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristie Lindsey Rose
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-02 21:24:41	ID requested
⏵ 1	2025-01-04 19:22:03	announced

Dataset with its publication pending

submitter keyword: ectosomes, microvesicles, large EVs, small EVs, Proteomics,Blebbisomes, LC-MS/MS

Kristie Lindsey Rose; Dennis Jeppesen
contact affiliation	Vanderbilt University Proteomics Core; Vanderbilt University Medical Center Department of Medicine
contact email	kristie.rose@vanderbilt.edu
lab head
Kristie Lindsey Rose
contact affiliation	Vanderbilt university
contact email	kristie.rose@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD059407

PRIDE project URI

• PRIDE
  1. PXD059407
     1. Label: PRIDE project
     2. Name: MDA-MB-231 blebbisome proteomics