PXD059391-1

PXD059391 is an original dataset announced via ProteomeXchange.

Title	Argonaute proteins regulate the timing of the spermatogenic transcriptional program
Description	Argonaute proteins are best known for their role in microRNA-mediated post-transcriptional gene silencing. Here, we show that AGO3, but not AGO2, localizes to the sex chromatin of pachytene spermatocytes nuclei, where together with AGO4, it is required for full transcriptional silencing of XY-linked genes. Using a triple Ago413-/- mouse, we show that AGO3 and AGO4 are key regulators of spermatogenesis, orchestrating program-wide expression of meiosis-related genes during prophase I, while maintaining silencing of spermiogenesis genes. Premature overexpression of spermiogenesis genes during prophase I in Ago413-/- mice results in subfertility, associated with severe spermiogenesis defects leading to altered sperm morphology and reduced fertilization capability. We also identify BRG1, a BAF complex subunit, as an AGO3 interactor. Loss of AGO3 and AGO4 results in increased BRG1 in spermatocytes, suggesting that AGO3 is needed to help remove BRG1 from the XY chromatin during meiotic sex chromosome inactivation and indicating a role for AGO3 in transcriptional control during meiosis through interaction with the chromatin remodeling machinery.
HostingRepository	PRIDE
AnnounceDate	2026-06-22
AnnouncementXML	Submission_2026-06-22_08:44:32.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qin Fu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-02 10:07:37	ID requested
⏵ 1	2026-06-22 08:44:33	announced

Dataset with its publication pending

submitter keyword: meiosis, Aargonaute , non-coding RNA, BRG1, meiotic sex chromosome inactivation, sex body, spermiogenesis

Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
lab head
Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059391
     1. Label: PRIDE project
     2. Name: Argonaute proteins regulate the timing of the spermatogenic transcriptional program