PXD059374-1

PXD059374 is an original dataset announced via ProteomeXchange.

Title	Microscope-Free Microdissection of Amyloid Plagues in Alzheimer’s Disease Tissue via Single Electron Transfer Photocatalysis
Description	Amyloid protein deposit, like Aβ plaques, is a pathological hallmark of Alzheimer’s disease (AD). While plenty of amyloid imaging reagents available for diagnosis, their composition associated with disease etiology is challenging to analyze in intact tissue sample. Herein, we report an amyloid-targeting probe to photocatalyze in-situ labeling and profiling of Aβ plaques in AD brain tissue via single electron transfer (SET) mechanism. First, we render this amyloid probe with photocatalytic labeling function by fragment fusion of classical scaffolds of fluorescent amyloid sensors. Second, we demonstrate its labeling selectivity to amyloid proteins over monomer counterparts. Mechanistically, we show spatially resolved labeling is driven by the short-lived SET photocatalytic process. Finally, we use this probe to capture and profile the composition of amyloid plagues in Alzheimer’s disease brain tissue. Surpassing other AD proteomics datasets, our work spatially resolves pathogenic Aβ and typical AD biomarkers as top-ranked hits. Together, the short-lived SET-driven photocatalysis spatially restrains protein labeling to occur in ultra-proximity to amyloids, allowing for microscope-free amyloid tissue microdissection at molecular level.
HostingRepository	PRIDE
AnnounceDate	2025-01-13
AnnouncementXML	Submission_2025-01-13_00:57:13.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	夏 秋璇
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-01 23:07:22	ID requested
⏵ 1	2025-01-13 00:57:14	announced

Dataset with its publication pending

submitter keyword: protein labeling,Alzheimer’s disease,single electron transfer,photocatalysis,photosensitizer

Yu Liu
contact affiliation	Dalian Institute of Chemical Physics
contact email	liuyu@dicp.ac.cn
lab head
夏 秋璇
contact affiliation	中国科学院大连化学物理研究所
contact email	1161134605@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059374
     1. Label: PRIDE project
     2. Name: Microscope-Free Microdissection of Amyloid Plagues in Alzheimer’s Disease Tissue via Single Electron Transfer Photocatalysis