PXD059270-1

PXD059270 is an original dataset announced via ProteomeXchange.

Title	Exploring the interplay between protein conformational changes and phosphorylation in a pancreatic cancer cell and stellate cell co-culture system
Description	A global exploration of the complex interplay between protein conformational changes and phosphorylation events in cell-cell interactions is crucial for understanding the dynamic nature of protein modifications. This understanding is essential for developing novel targeted therapies for pancreatic cancer, particularly in the context of interactions between pancreatic cancer cells (PCCs) and pancreatic stellate cells (PSCs). However, protein conformational changes that occur during PCCs-PSCs interactions remain poorly studied, and the relationship between these changes and phosphorylation is not well understood. Here, we present a comprehensive mass spectrometry-based study investigating the interplay between protein conformational alterations and phosphorylation in a co-culture system of pancreatic ductal adenocarcinoma cells (PANC-1) and PSCs. Our results demonstrate that 435 proteins exhibiting conformational changes were detected during the co-culture of PANC-1 with PSCs, primarily involving proteins associated with the tricarboxylic acid (TCA) cycle, glycolysis/gluconeogenesis, and carbon metabolism. Our findings also highlight a potential association between phosphorylation and protein conformational changes. Moreover, we identified five potential conformational targets, including ACLY, ACO1, ACO2, IDH1, and OGDH, which may provide valuable insights into the molecular pathways underlying gemcitabine resistance in pancreatic cancer. Overall, these results offer novel insights into protein conformational changes and their potential link to phosphorylation in the context of cancer-stromal cell interactions, paving the way for structure-based, targeted therapeutic strategies for pancreatic cancer treatment.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-15_10:58:07.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haiyan Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-26 22:16:01	ID requested
⏵ 1	2025-12-15 10:58:08	announced

Dataset with its publication pending

submitter keyword: None

Lingjun Li
contact affiliation	University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Haiyan Lu
contact affiliation	University of Wisconsin-Madison
contact email	luhaiyan2017@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059270
     1. Label: PRIDE project
     2. Name: Exploring the interplay between protein conformational changes and phosphorylation in a pancreatic cancer cell and stellate cell co-culture system