PXD059239-1

PXD059239 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Selective Enrichment of Newly Synthesized Proteins using Phos-tag Click Tip Enables Nascent Proteome Analysis in Influenza A Virus Infection
Description	Bioorthogonal non-canonical amino acid tagging (BONCAT)-based approaches enable selective labeling of newly synthesized proteins (NSPs). We developed a workflow termed the “Phos-tag Click Tip” by integrating a phosphorylated variant of bicyclononyne (pBCN) with Phos-tag affinity purification to selectively capture azidohomoalanine (AHA)-labeled peptides. Applying this workflow, we performed comprehensive NSP profiling of host cells during influenza A virus (IAV) infection. We identified dynamic changes in NSP profiles associated with viral replication, host restriction, and immune responses, many of which were not readily detected by conventional whole-cell or phosphoproteomic analyses.
HostingRepository	jPOST
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-08_18:40:50.770.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yayoi Kimura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	unknown modification; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification; unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-24 21:48:41	ID requested
⏵ 1	2026-09-08 18:40:51	announced

Publication List

Kimura Y, Akiyama T, Kobashi T, Hosotani M, Ino Y, Shin J, Nakamura N, Kinoshita E, Miyakawa K, Ryo A, Selective Enrichment of Newly Synthesized Proteins Using Phos-Tag Click Tip Enables Nascent Proteome Analysis in Influenza A Virus Infection. J Proteome Res, 25(9):4888-4902(2026) [pubmed]

Kimura Y, Akiyama T, Kobashi T, Hosotani M, Ino Y, Shin J, Nakamura N, Kinoshita E, Miyakawa K, Ryo A, Selective Enrichment of Newly Synthesized Proteins Using Phos-Tag Click Tip Enables Nascent Proteome Analysis in Influenza A Virus Infection. J Proteome Res, 25(9):4888-4902(2026) [pubmed]

Keyword List

submitter keyword: Azidohomoalanine, BONCAT,Newly synthesized protein, Influenza A virus

submitter keyword: Azidohomoalanine, BONCAT,Newly synthesized protein, Influenza A virus

Contact List

Yayoi Kimura
lab head
Yayoi Kimura
contact affiliation	Yokohama City University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003525/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003525/