PXD059213-1

PXD059213 is an original dataset announced via ProteomeXchange.

Title	Illumina NovaSeq 6000 Mannose-B from Codonopsis Pilosula Modulates LAMB1 Expression to Enhance Trophoblast Function and Alleviate Subchorionic Hematoma
Description	Introduction: This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Methods: Key genes involved in SCH pathology were identified through RNA sequencing and bioinformatics analysis. Network pharmacology was utilized to screen active components in Codonopsis pilosula and their critical targets. In vitro, HTR-8/Svneo cells were used to assess proliferation, migration, and invasion through CCK8, Transwell, and cell migration assays. A SCH rat model was established to evaluate changes in coagulation parameters, litter size, fetal viability, and fetal and placental weights. In vivo validation of Mannose-B's effects on LAMB1 expression and SCH pathology was performed using RT-qPCR and Western Blot. Results: Network pharmacology and molecular docking identified Mannose-B as an effective compound in Codonopsis pilosula, potentially beneficial for SCH treatment, with LAMB1 as a significant target. In vitro experiments showed that Mannose-B enhanced HTR-8/Svneo cell proliferation, migration, and invasion by reducing LAMB1 expression. In vivo experiments confirmed Mannose-B's inhibitory effect on placental LAMB1 expression and its potential in ameliorating SCH pathology. Conclusion: Mannose-B from Codonopsis pilosula inhibits LAMB1 expression, promoting human placental trophoblast cell proliferation, migration, and invasion, thereby mitigating the progression of SCH pathology.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_16:16:22.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-24 03:32:34	ID requested
⏵ 1	2025-10-19 16:16:23	announced

Sun Q, Gao Y, Asan K, Ji J, Xu J, Wu X, Ren J, Feng W, Song M, Wang S, Zhang B, Zhou Y, Han C, Liu S, Yang W, Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma. Sci Rep, 15(1):29297(2025) [pubmed]

10.1038/s41598-025-13918-z;

submitter keyword: Subchorionic Hematoma

Codonopsis Pilosula

Mannose-B

Laminin Subunit Beta 1

Trophoblast Cells

Network Pharmacology

Sun Qian
contact affiliation	The First People's Hospital of Lianyungang
contact email	sunqian0818@126.com
lab head
Qian Sun
contact affiliation	The First People's Hospital of Lianyungang
contact email	sunqian0818@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059213
     1. Label: PRIDE project
     2. Name: Illumina NovaSeq 6000 Mannose-B from Codonopsis Pilosula Modulates LAMB1 Expression to Enhance Trophoblast Function and Alleviate Subchorionic Hematoma