PXD059096 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Breaking Barriers in Crosslinking Mass Spectrometry: Enhanced Throughput and Sensitivity with the Orbitrap Astral Mass Analyzer |
| Description | Crosslinking mass spectrometry (XL-MS) has become a key technique for investigating protein-protein interactions and the architecture of biological systems. This study compares the Orbitrap Astral and Eclipse mass spectrometers in XL-MS workflows, emphasizing their performance in detecting low-abundance crosslinked peptides. Using Cas9 crosslinked with PhoX and DSSO, the Astral demonstrated over 40% higher crosslink identifications due to its superior ion transmission and sensitivity, enabled by its multi-reflection time-of-flight analyzer. Single higher-energy collisional dissociation (HCD) fragmentation was more effective than stepped HCD on the Astral, while the Eclipse showed similar results for both methods. Gradient optimization revealed that extended separation times improved crosslink identifications. These findings highlight the critical role of instrumentation and workflow optimization in advancing XL-MS for structural proteomics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-16 |
| AnnouncementXML | Submission_2025-12-15_23:14:29.176.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Fränze Müller |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse; Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-20 06:24:24 | ID requested | |
| ⏵ 1 | 2025-12-15 23:14:29 | announced | |
Publication List
Keyword List
| submitter keyword: crosslinking mass spectrometry, fragmentation, Orbitrap Eclipse, HCD, quality control, compensation voltage, Orbitrap Astral |
Contact List
| Karl Mechtler |
|---|
| contact affiliation | Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria. |
| contact email | karl.mechtler@imp.ac.at |
| lab head | |
| Fränze Müller |
|---|
| contact affiliation | Research Institute of Molecular Pathology (IMP), VBCF Vienna |
| contact email | fraenze.mueller@gmx.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD059096 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059096
- Label: PRIDE project
- Name: Breaking Barriers in Crosslinking Mass Spectrometry: Enhanced Throughput and Sensitivity with the Orbitrap Astral Mass Analyzer
