PXD059093-1

PXD059093 is an original dataset announced via ProteomeXchange.

Title	Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance
Description	The presentation of peptides on HLA molecules is essential to CD8+ T cell responses. Here, we show that loss of uL14 significantly downregulates the expression of antigen processing and presentation (APP) components in melanoma cell lines. Peptides generated following knock down show different characteristics, with altered peptide charge, and differences in anchor residue positions. These peptides also have lower predicted binding to the HLA alleles, and a shorter predicted HLA-peptide complex half-life. These result in a functional difference in APPand knock down of uL14 causes a reduction in the ability of CD8+ T cells to recognize and kill melanoma cells in a co-culture assay. Together, our data suggests that loss of uL14 alters the peptide pool available for presentation and thus may act as an escape mechanism from tumor immune surveillance.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_23:38:58.472.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liesbeth Hoekman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-20 05:16:15	ID requested
⏵ 1	2025-09-14 23:38:59	announced

10.1093/narcan/zcaf024;

Dopler A, Kyei-Baffour ES, Kerkhoff M, Alkan F, Malka Y, Hoefakker K, van der Kammen R, Hoekman L, Bleijerveld O, Bradaric A, Altelaar M, Yewdell JW, Kvistborg P, Faller WJ, cell immunosurveillance. NAR Cancer, 7(3):zcaf024(2025) [pubmed]

submitter keyword: LC-MS/MS,Ribosome

uL14

Antigen presentation

Onno Bleijerveld
contact affiliation	Mass Spectrometry/Proteomics Facility, Netherlands Cancer Institute, Amsterdam, Netherlands
contact email	O.bleijerveld@nki.nl
lab head
Liesbeth Hoekman
contact affiliation	The Netherlands Cancer Institute, Amsterdam, The Netherlands.
contact email	l.hoekman@nki.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD059093

PRIDE project URI

• PRIDE
  1. PXD059093
     1. Label: PRIDE project
     2. Name: Loss of ribosomal protein uL14 enables tumor escape from T cell immunosurveillance