PXD059082-1

PXD059082 is an original dataset announced via ProteomeXchange.

Title	Systemic factors from young human serum rejuvenate human skin in vitro using a microphysiological system featuring BM-derived blood cells
Description	Aging is a multifaceted systemic process that contributes to the onset of age-related diseases. Despite advances, few comprehensive anti-aging strategies have successfully reversed the adverse effects of aging. Heterochronic parabiosis studies have highlighted the potential of systemic rejuvenation through blood-borne factors, yet the precise drivers of aging and rejuvenation, along with their mechanisms, remain largely elusive. Furthermore, translating these findings to humans has been challenging. In this study, we achieved human skin rejuvenation through systemic factors using a microphysiological system comprising a 3D skin model and a 3D bone marrow model, which simulates the niche for progenitor and blood cells. Treatment with young human serum, compared to aged human serum, enhanced cell proliferation and reduced the biological age of skin tissue, as determined by methylation-based age clocks. Notably, these effects required the presence of bone marrow-derived cells in the system. Further analysis of the bone marrow model revealed serum-dependent changes in cell population composition and aging markers. Proteome profiling identified 55 potential systemic rejuvenating proteins secreted by bone marrow-derived cells. Among these, seven proteins were validated to have rejuvenating effects on human skin cells through hallmark aging assays, underscoring their role as key systemic factors in reversing skin aging.
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_06:43:36.074.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-20 01:40:49	ID requested
⏵ 1	2025-07-29 06:43:36	announced

10.18632/AGING.206288;

submitter keyword: bone marrow, skin, mesenchymal stem cells,aging

Stefan Kalkhof
contact affiliation	Institute for Bioanalytics, Coburg University of Applied Sciences and Arts, Coburg, Germany
contact email	stefan.kalkhof@hs-coburg.de
lab head
Johannes Schmidt
contact affiliation	Fraunhofer Institute for Cell Therapy and Immunology
contact email	johannes.schmidt@izi.fraunhofer.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059082

PRIDE project URI

• PRIDE
  1. PXD059082
     1. Label: PRIDE project
     2. Name: Systemic factors from young human serum rejuvenate human skin in vitro using a microphysiological system featuring BM-derived blood cells