PXD059070-1

PXD059070 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial stress drives skeletal muscle remodelling following sprint-interval exercise.
Description	Mitochondria play a central role in cellular metabolism and are crucial for maintaining cellular health. Exercise is a proven intervention to enhance mitochondrial function and prevent non-communicable diseases, yet the molecular mechanisms driving exercise-induced mitochondrial remodelling remain unclear. This study compares the effects of moderate-intensity continuous exercise (MICE) and sprint-interval exercise (SIE) on mitochondrial adaptations. We demonstrate that SIE induces mitochondrial stress and activates the mitochondrial unfolded protein response (UPRmt), alongside disturbances in mitochondrial structure while MICE promotes mitochondrial adaptations characterized by increased mitochondrial content. Over an 8-week training period, moderate-intensity continuous training (MICT) and sprint-interval training (SIT) show divergent mitochondrial remodelling. Findings provide novel insights into the mechanisms by which different exercise prescriptions modulate mitochondrial signalling and remodelling in human skeletal muscle, offering a basis for personalized exercise strategies to optimize mitochondrial health.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_18:54:13.861.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Stroud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-19 22:19:37	ID requested
⏵ 1	2026-01-11 18:54:14	announced

10.1038/s41467-025-66625-8;

Botella J, Perri E, Caruana NJ, L, ó, pez-Calcerrada S, Brischigliaro M, Jamnick NA, Oorschot V, Saner NJ, D, í, az-Lara J, Taylor DF, Garnham A, Fern, á, ndez-Vizarra E, Ugalde C, Ramm G, Stroud DA, Lazarou M, Bishop DJ, Sprint interval exercise disrupts mitochondrial ultrastructure driving a unique mitochondrial stress response and remodelling in men. Nat Commun, 17(1):71(2025) [pubmed]

submitter keyword: Exercise,Mitochondria, Muscle, DIA, Human

David Stroud
contact affiliation	Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, 3010, Australia 2Murdoch Children’s Research Institute, Melbourne, VIC 3052, Australia 3Victorian Clinical Genetics Services, Murdoch Children’s Research Institute, Melbourne, VIC, 3052, Australia
contact email	david.stroud@unimelb.edu.au
lab head
David Stroud
contact affiliation	The University of Melbourne
contact email	david.stroud@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD059070

PRIDE project URI

• PRIDE
  1. PXD059070
     1. Label: PRIDE project
     2. Name: Mitochondrial stress drives skeletal muscle remodelling following sprint-interval exercise.