PXD059065 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantification of the percentage of oxidized cysteine thiols in GrxS12 under normal and H2O2 treatments. |
| Description | To evaluate the percentage of oxidized cysteine thiols, the GrxS12 proteins underwent either with or without H2O2 treatment were alkylated by iodoacetamide (IAM), reduced with DTT, and subsequently the newly generated free Cys were alkylated with N-ethylmaleimide (NEM). The spectrometric analysis of the GrxS12 sample indicated that less than 30% of monomers formed an intramolecular disulfide linkage. This confirmed the formation of intramolecular disulfide bonds between Cys34 and Cys92 and demonstrated that the percentage of intramolecular disulfide linkage remained unaltered under normal and H₂O₂- oxidized conditions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-16 |
| AnnouncementXML | Submission_2026-01-15_18:23:52.590.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yanshuang Liu |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | dihydroxylated residue; N-ethylmaleimide derivatized cysteine; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-19 19:46:49 | ID requested | |
| ⏵ 1 | 2026-01-15 18:23:53 | announced | |
Publication List
Keyword List
| submitter keyword: GrxS12, H2O2, oxidized cysteine thiols, quantification |
Contact List
| Shaojun Dai |
|---|
| contact affiliation | Shanghai Normal University,Shanghai,China |
| contact email | daishaojun@shnu.edu.cn |
| lab head | |
| Yanshuang Liu |
|---|
| contact affiliation | Shanghai Normal University |
| contact email | 920662600@qq.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD059065 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059065
- Label: PRIDE project
- Name: Quantification of the percentage of oxidized cysteine thiols in GrxS12 under normal and H2O2 treatments.
