PXD059029-1

PXD059029 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein expression in warmed human lens epithelium cells using shotgun proteomics
Description	In previous studies, we reported that cumulative thermal dose estimation in crystalline lens, as determined through computer simulation analysis using a supercomputer and a biothermal transport equation, showed a strong correlation with the prevalence of nuclear cataracts. In this study, we have investigated what kind of protein expressed underlying normal (35.0 °C) and warming conditions (37.5 °C) by using the shotgun Liquid chromatography with tandem mass spectrometry (LC-MS/MS)-based global proteomic approach. A total of 615 proteins were identified in the iHLEC-NY2, including 307 (49.9%) present in both lens cultured at 35.0 °C and 37.5 °C, 130 (21.1%) unique to lens cultured at 35.0 °C, and 178 (29.0%) unique to lens cultured at 37.5 °C. Furthermore, we have discussed the changes in protein expression in warmed iHLEC-NY2 cells using Gene Ontology analysis and a label-free semiquantitative method based on spectral counting. Shotgun proteomic analysis revealed that warming decreased the expression of actin, alpha cardiac muscle 1, actin-related protein 2, putative tubulin-like protein alpha-4B, ubiquitin carboxyl-terminal hydrolase 17-like protein 1, ubiquitin-ribosomal protein eL40 fusion protein, ribosome biogenesis protein BMS1 homolog, histone H2B type 1-M, and histone H2A.J. in iHLEC-NY2. Thus, the decrease in specific protein levels of actin, tubulin, ubiquitin, ribosomes, and histones may be related to cataract development under warming conditions. This study may serve as a valuable indicator for elucidating the relationship between temperature and onset of nuclear cataracts.
HostingRepository	jPOST
AnnounceDate	2025-12-19
AnnouncementXML	Submission_2025-12-18_07:00:05.149.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroko Otake
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 21:33:01	ID requested
⏵ 1	2025-12-18 07:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: immortalized human lens epithelium cell, temperature, shotgun proteomics, cataract, protein expression

submitter keyword: immortalized human lens epithelium cell, temperature, shotgun proteomics, cataract, protein expression

Contact List

Noriaki Nagai
lab head
Hiroko Otake
contact affiliation	Kindai University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003507/