PXD059014-1

PXD059014 is an original dataset announced via ProteomeXchange.

Title	Cytonuclear Fractionation TPCA in HSV-1 RF Infection
Description	We developed a cytonuclear fractionated TPCA workflow, enabling the probing of global protein-protein interaction (PPI) networks with spatial resolution. We show that fractionation improves downstream PPI prediction, as well as increases the number of PPIs that can be detected. We apply this new workflow to study the spatiotemporal dynamics of host and viral proteins during infection with a mutant strain of HSV-1, HSV-1 RF.
HostingRepository	PRIDE
AnnounceDate	2025-07-07
AnnouncementXML	Submission_2025-07-07_07:22:09.144.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059014
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tavis Reed
SpeciesList	scientific name: Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1); NCBI TaxID: 10299; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 15:40:14	ID requested
⏵ 1	2025-07-07 07:22:10	announced

10.6019/PXD059014;

Metzger PJ, Reed TJ, Lum KK, Botello JF, Jiang L, Brangwynne CP, Troyanskaya OG, Cristea IM, Sequestration of ribosome biogenesis factors in HSV-1 nuclear aggregates revealed by spatially resolved thermal profiling. Sci Adv, 11(26):eadw6814(2025) [pubmed]

10.1126/sciadv.adw6814;

submitter keyword: Human, TPCA, Subcellular Fractionation, HSV-1

Ileana M. Cristea
contact affiliation	Department of Molecular Biology, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Tavis Reed
contact affiliation	Princeton University
contact email	tjreed@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD059014

PRIDE project URI

• PRIDE
  1. PXD059014
     1. Label: PRIDE project
     2. Name: Cytonuclear Fractionation TPCA in HSV-1 RF Infection