PXD059008-1

PXD059008 is an original dataset announced via ProteomeXchange.

Title	Low glucose culture conditions alter neuronal energetics, mimicking in vivo studies
Description	Neurons are almost exclusively cultured in media containing glucose at much higher concentration than found in the brain. To test whether “standard” hyperglycaemic culture conditions affect neuronal respiration compared to near- euglycemic conditions, we grew neuronal cultures with minimal glial contamination from hippocampus and cortex of neonatal C57bl6 mice in standard commercially available media (25 mM Glucose) and in identical media with 5 mM glucose. Neurons grew well in both glucose concentrations until at least 14 days in vitro, with similar morphology and synaptogenesis. Neurons grown in high glucose were more dependent on glycolysis as their primary source of ATP, measured using ATP luminescence and cellular respirometry assays. In contrast, neurons grown in 5 mM glucose showed a more balanced dependence on glycolysis and mitochondrial oxidative phosphorylation (OXPHOS) and greater reserve mitochondrial respiration capacity, relative to standard media. Changes in gene and protein expression levels corroborate these changes in function. Our results suggest that neurons cultured in high glucose media preferentially use glycolysis, opposite to what is known for neurons in vivo as the primary pathway for ATP maintenance. We suggest using neuronal culture systems in 5 mM glucose to better represent physiologically relevant neuronal respiration.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:23:05.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juli Petereit
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 12:32:04	ID requested
⏵ 1	2025-06-29 16:23:06	announced

Swain S, Roberts DM, Chowdhry S, Durbin R, Boyd R, Petereit J, Renden R, Low-Glucose Culture Conditions Bias Neuronal Energetics Towards Oxidative Phosphorylation. J Neurochem, 169(6):e70125(2025) [pubmed]

10.1111/jnc.70125;

submitter keyword: Primary mouse neuron, Cell culture, Glucose, mitochondrial respiration, Neurobasal Media,Neuronal Bioenergetics

Robert Renden
contact affiliation	University of Neada, Reno
contact email	rendenr@unr.edu
lab head
Juli Petereit
contact affiliation	University of Nevada, Reno
contact email	nbc@unr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD059008

PRIDE project URI

• PRIDE
  1. PXD059008
     1. Label: PRIDE project
     2. Name: Low glucose culture conditions alter neuronal energetics, mimicking in vivo studies