PXD058985-1

PXD058985 is an original dataset announced via ProteomeXchange.

Title	The related EIF4G3 and EIF4G4 initiation factors from Leishmania: dissimilar modes of action during translation revealed by a comparative proteomic approach
Description	mRNA translation in eukaryotes starts with the binding of the eIF4F complex to its capped 5' end. eIF4F is formed by the joining of its eIF4G and eIF4E subunits, with the former mediating relevant interactions required for translation to proceed. Five eIF4G homologues are found in Leishmania, as part of multiple eIF4F-like complexes, all likely active in translation. Here we performed a large-scale comparative analysis of protein partners associated with two of these eIF4Gs, the related EIF4G3 and EIF4G4. Different transgenic Leishmania infantum cell lines were generated expressing these proteins tagged with a C-terminal HA epitope, each capable of complementing the deletion of the native protein. Proteins associated with the HA-tagged eIF4Gs, after immunoprecipitation with anti-HA beads, were identified through mass spectrometry from three different growth phases: early exponential (24hs), late exponential (48hs) and stationary (96hs). EIF4G3 and EIF4G4 co-precipitated with known and novel protein partners belonging to different functional categories, with some restricted to selected growth phases. The results are thus compatible with the two eIF4Gs likely having related but distinct functions during translation and possibly being targeted by different regulatory mechanisms which are affected by cell growth.
HostingRepository	PRIDE
AnnounceDate	2026-04-13
AnnouncementXML	Submission_2026-04-12_16:23:36.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stéphanny Oliveira
SpeciesList	scientific name: Leishmania infantum JPCM5; NCBI TaxID: NEWT:435258;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 05:55:37	ID requested
⏵ 1	2026-04-12 16:23:37	announced

10.1186/s13071-026-07297-1;

Oliveira SSS, Melo do Nascimento L, Moura DMN, de Souza Reis CR, de Melo Neto OP, The related EIF4G3 and EIF4G4 initiation factors from Leishmania: dissimilar modes of action during translation revealed by a comparative proteomic approach. Parasit Vectors, 19(1):(2026) [pubmed]

submitter keyword: protein synthesis, gene expression regulation, trypanosomatid

Osvaldo Pompílio de Melo Neto
contact affiliation	Microbiology departament, Aggeu Magalhães Institute, Brazil.
contact email	osvaldo.pompilio@fiocruz.br
lab head
Stéphanny Oliveira
contact affiliation	Instituo Aggeu Magalhães
contact email	stephannys@fiocruz.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058985
     1. Label: PRIDE project
     2. Name: The related EIF4G3 and EIF4G4 initiation factors from Leishmania: dissimilar modes of action during translation revealed by a comparative proteomic approach